Rabbit anti-Human SPHK1 Polyclonal Antibody | anti-SPHK1 antibody

SPHK1 (Sphingosine Kinase 1, SK 1, SPHK, SPK 1, SPK) (HRP)

Cat. Num. AAA6394909

• Gene Names: SPHK1; SPHK
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SPHK1; Polyclonal Antibody; SPHK1 (Sphingosine Kinase 1; SK 1; SPHK; SPK 1; SPK) (HRP); EC=2.7.1.91; anti-SPHK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SPHK1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SPHK1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human SPHK1, aa1-398 (NP_068807.2).
• Immunogen Sequence: MDPVVGCGRGLFGFVFSAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of SPHK1 expression in transfected 293T cell line by SPHK1 polyclonal antibody. Lane 1: SPHK1 transfected lysate (43.9kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-SPHK1 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 21361088
• NCBI GeneID: 8877
• NCBI Accession #: NP_068807.2
• NCBI GenBank Nucleotide #: NM_021972
• UniProt Accession #: Q53ZR5; Q96GK1; Q96HV8; Q9BTG7; Q9NYA1
• Molecular Weight: 42,518 Da
• NCBI Official Full Name: sphingosine kinase 1 isoform 1
• NCBI Official Synonym Full Names: sphingosine kinase 1
• NCBI Official Symbol: SPHK1
• NCBI Official Synonym Symbols: SPHK
• NCBI Protein Information: sphingosine kinase 1; SK 1; SPK 1
• UniProt Protein Name: Sphingosine kinase 1
• UniProt Gene Name: SPHK1
• UniProt Entry Name: Q53ZR5_HUMAN

NCBI Description

Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM]

Uniprot Description

SPHK1: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Interacts with ACY1. Binds to calmodulin. Interacts with SPHKAP. Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, lipid; Lipid Metabolism - sphingolipid; EC 2.7.1.91

Chromosomal Location of Human Ortholog: 17q25.2

Cellular Component: synaptic vesicle; axon; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: D-erythro-sphingosine kinase activity; calmodulin binding; protein binding; DNA binding; protein phosphatase 2A binding; sphinganine kinase activity; magnesium ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: protein folding; positive regulation of NF-kappaB import into nucleus; female pregnancy; signal transduction; positive regulation of mitotic cell cycle; positive regulation of neurotransmitter secretion; activation of NF-kappaB transcription factor; 'de novo' posttranslational protein folding; positive regulation of fibroblast proliferation; response to magnesium ion; regulation of interleukin-1 beta production; protein kinase C activation; positive regulation of smooth muscle contraction; inflammatory response; blood vessel development; sphingolipid metabolic process; sphingolipid biosynthetic process; cyclooxygenase pathway; calcium-mediated signaling; sphingosine metabolic process; lipid phosphorylation; cellular response to starvation; positive regulation of cell growth; response to ATP; positive regulation of angiogenesis; cellular protein metabolic process; positive regulation of protein ubiquitination; brain development; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; response to progesterone stimulus; negative regulation of apoptosis; response to amine stimulus; positive regulation of cell migration; sphingoid catabolic process