Western Blot (WB)
(SPDYE5 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the SPDYE5 antibody detected the SPDYE5 protein (arrow).)
Rabbit anti-Human SPDYE5 Polyclonal Antibody | anti-SPDYE5 antibody
SPDYE5 Antibody (N-term)
Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SPDYE5; Polyclonal Antibody; SPDYE5 Antibody (N-term); Putative speedy protein E5; anti-SPDYE5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SPDYE5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human SPDYE5.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
21-50
Applicable Applications for anti-SPDYE5 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
FC~~1:10~1:50
FC~~1:10~1:50
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(SPDYE5 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the SPDYE5 antibody detected the SPDYE5 protein (arrow).)
Western Blot (WB)
(SPDYE5 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the SPDYE5 antibody detected the SPDYE5 protein (arrow).)
Flow Cytometry (FC/FACS)
(SPDYE5 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(SPDYE5 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-SPDYE5 antibody
References
Hillier, L.W., et al. Nature 424(6945):157-164(2003)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47997
NCBI Official Synonym Full Names
speedy/RINGO cell cycle regulator family member E5
NCBI Official Symbol
SPDYE5
NCBI Protein Information
putative speedy protein E5
UniProt Protein Name
Putative speedy protein E5
Protein Family
UniProt Gene Name
SPDYE5
UniProt Entry Name
SPDE5_HUMAN
Uniprot Description
SPDYE5: Belongs to the Speedy/Ringo family.
Chromosomal Location of Human Ortholog: 7q11.23
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Product Notes
The SPDYE5 spdye5 (Catalog #AAA9207394) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 21-50. The SPDYE5 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPDYE5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS). WB~~1:1000 FC~~1:10~1:50. Researchers should empirically determine the suitability of the SPDYE5 spdye5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPDYE5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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