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Western Blot (WB) (Western blot detection of RanBP9 in Jurkat, MOLT-4 and CEM cell lysates and using RanBP9 mouse mAb (1:1000 diluted).Predicted band size: 78KDa.Observed band size: 95KDa.)

anti-Human RanBP9 Monoclonal Antibody | anti-RanBP9 antibody

Anti-RanBP9 Mouse mAb

Gene Names
RANBP9; BPM-L; BPM90; RANBPM; RanBP7
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunoprecipitation
Purity
Affinity Purified
Synonyms
RanBP9; Monoclonal Antibody; Anti-RanBP9 Mouse mAb; B cell antigen receptor Ig beta associated protein 1; BPM 90; BPM L; BPM-L; BPM90; BPML; IBAP 1; Imp 9; Importin 9; Novel centrosomal protein RanBPM; RAN binding protein 9; Ran binding protein centrosomal; Ran Binding Protein in the Microtubule organizing center; Ran binding protein M; Ran BP9; Ran-binding protein 9; Ran-binding protein M; RANB9_HUMAN; RanBP 7; RANBP 9; RanBP7; RanBPM; anti-RanBP9 antibody
Ordering
For Research Use Only!
Host
Mouse
Source: Human
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3C3-E11-C12
Purity/Purification
Affinity Purified
Form/Format
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%, glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
729
Applicable Applications for anti-RanBP9 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 1:1000
ICC: 1:300
Immunogen
Purified recombinant human RanBP9 protein fragments expressed in E Coli.
Sensitivity
This antibody detects endogenous levels of RanBP9 and does not cross-react with related proteins.
Sequence Similarities
Belongs to the RANBP9/10 family.Contains 1 B30.2/SPRY domain.Contains 1 CTLH domain.Contains 1 LisH domain.
Subunit Structure
Interacts with NGFR and DDX4. Interacts with GTP-bound Ran, AR, CDC2L1/p110C, CALB1, S100A7, USP11, MKLN1, SOS1 or SOS2, C20orf11, and FMR1. Interacts with the Dyrk kinases HIPK2, DYRK1A, and DYRK1B. Interacts with TP73 isoform Alpha but not with TP53. Interacts with the HGF receptor MET and the integrins ITGB1 and ITGB2, but not with ITGAL. Part of a complex consisting of RANBP9, MKLN1 and C20ORF11. Part of a complex consisting of RANBP9, RAN, DYRK1B and COPS5. Directly interacts with RANBP10.
Subcellular Location
Cytoplasm; cytosol. Nucleus.

Western Blot (WB)

(Western blot detection of RanBP9 in Jurkat, MOLT-4 and CEM cell lysates and using RanBP9 mouse mAb (1:1000 diluted).Predicted band size: 78KDa.Observed band size: 95KDa.)

Western Blot (WB) (Western blot detection of RanBP9 in Jurkat, MOLT-4 and CEM cell lysates and using RanBP9 mouse mAb (1:1000 diluted).Predicted band size: 78KDa.Observed band size: 95KDa.)

Immunocytochemistry (ICC)

(Immunocytochemistry stain of Hela using RanBP9 mouse mAb (1:300).)

Immunocytochemistry (ICC) (Immunocytochemistry stain of Hela using RanBP9 mouse mAb (1:300).)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of Hela cell lysates using RanBP9 mouse mAb.)

Immunoprecipitation (IP) (Immunoprecipitation analysis of Hela cell lysates using RanBP9 mouse mAb.)
Related Product Information for anti-RanBP9 antibody
Entrez Summary: This gene encodes a protein that binds RAN, a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The protein encoded by this gene has also been shown to interact with several other proteins, including met proto-oncogene, homeodomain interacting protein kinase 2, androgen receptor, and cyclin-dependent kinase 11.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
78
NCBI Official Full Name
ran-binding protein 9
NCBI Official Synonym Full Names
RAN binding protein 9
NCBI Official Symbol
RANBP9
NCBI Official Synonym Symbols
BPM-L; BPM90; RANBPM; RanBP7
NCBI Protein Information
ran-binding protein 9
UniProt Protein Name
Ran-binding protein 9
Protein Family
UniProt Gene Name
RANBP9
UniProt Synonym Gene Names
RANBPM; RanBP9; RanBPM

NCBI Description

This gene encodes a protein that binds RAN, a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The protein encoded by this gene has also been shown to interact with several other proteins, including met proto-oncogene, homeodomain interacting protein kinase 2, androgen receptor, and cyclin-dependent kinase 11. [provided by RefSeq, Jul 2008]

Uniprot Description

RANBP9: May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. May be involved in signaling of ITGB2/LFA-1 and other integrins. Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but not affect estrogen-induced transactivation. Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity. Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA. Belongs to the RANBP9/10 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; GAPs; GAPs, Ras; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 6p23

Cellular Component: cytoplasm; cytosol; microtubule associated complex; nucleus

Molecular Function: enzyme binding; protein binding; Ran GTPase binding

Biological Process: axon guidance; protein complex assembly

Research Articles on RanBP9

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Product Notes

The RanBP9 ranbp9 (Catalog #AAA475103) is an Antibody produced from Mouse Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-RanBP9 Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RanBP9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 1:1000 ICC: 1:300. Researchers should empirically determine the suitability of the RanBP9 ranbp9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RanBP9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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