Rabbit SPAST Polyclonal Antibody | anti-SPAST antibody

SPAST Antibody

Cat. Num. AAA9403655

• Gene Names: SPAST; FSP2; SPG4; ADPSP
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Antigen affinity purification.
• Synonyms: SPAST; Polyclonal Antibody; SPAST Antibody; FSP2; SPG4; ADPSP; anti-SPAST antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total SPAST protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
• Concentration: 1.3 mg/ml (varies by lot)
• Sequence Length: 616

• Applicable Applications for anti-SPAST antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:25-1:100
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide corresponding to residues near the C terminal of human spastin
• Target Name: SPAST
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/20.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human brain tissue using at dilution 1/20.)

Related Product Information for anti-SPAST antibody

This gene encodes a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. The encoded ATPase may be involved in the assembly or function of nuclear protein complexes. Two transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their full length sequences have not been determined. Mutations associated with this gene cause the most frequent form of autosomal dominant spastic paraplegia 4.

Product Categories/Family for anti-SPAST antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 11875211
• NCBI GeneID: 6683
• NCBI Accession #: NP_055761.2
• NCBI GenBank Nucleotide #: NM_014946.3
• UniProt Accession #: Q9UBP0; Q9UPR9; A7E2A7
• Molecular Weight: 54,418 Da
• NCBI Official Full Name: spastin isoform 1
• NCBI Official Synonym Full Names: spastin
• NCBI Official Symbol: SPAST
• NCBI Official Synonym Symbols: FSP2; SPG4; ADPSP
• NCBI Protein Information: spastin
• UniProt Protein Name: Spastin
• Protein Family: Spastin
• UniProt Gene Name: SPAST
• UniProt Entry Name: SPAST_HUMAN

NCBI Description

This gene encodes a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. The encoded ATPase may be involved in the assembly or function of nuclear protein complexes. Two transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their full length sequences have not been determined. Mutations associated with this gene cause the most frequent form of autosomal dominant spastic paraplegia 4. [provided by RefSeq, Jul 2008]

Uniprot Description

spastin: ATP-dependent microtubule severing protein. Microtubule severing may promote reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and for completion of the abscission stage of cytokinesis. May also play a role in axon growth and the formation of axonal branches. Defects in SPAST are the cause of spastic paraplegia autosomal dominant type 4 (SPG4). Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. SPG4 is the most common form of autosomal dominant spastic paraplegias. Belongs to the AAA ATPase family. Spastin subfamily. 4 isoforms of the human protein are produced by alternative promoter.

Protein type: Membrane protein, integral; Cytoskeletal; EC 3.6.4.3

Chromosomal Location of Human Ortholog: 2p24-p21

Cellular Component: microtubule cytoskeleton; microtubule; centrosome; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; integral to membrane; spindle; cytoplasmic vesicle; midbody; nucleus; endosome

Molecular Function: protein binding; microtubule binding; beta-tubulin binding; microtubule-severing ATPase activity; ATP binding; alpha-tubulin binding

Biological Process: ER to Golgi vesicle-mediated transport; positive regulation of microtubule depolymerization; axonogenesis; metabolic process; microtubule severing; cytoplasmic microtubule organization and biogenesis; microtubule bundle formation; protein homooligomerization

Disease: Spastic Paraplegia 4, Autosomal Dominant