Rabbit anti-Human SPARCL1 Polyclonal Antibody | anti-SPARCL1 antibody

SPARCL1, ID (SPARCL1, SPARC-like protein 1, High endothelial venule protein, MAST 9) (Biotin)

Cat. Num. AAA6350323

• Gene Names: SPARCL1; SC1; MAST9; PIG33; MAST 9
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SPARCL1; Polyclonal Antibody; ID (SPARCL1; SPARC-like protein 1; High endothelial venule protein; MAST 9) (Biotin); MAST 9; anti-SPARCL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-SPARCL1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SPARCL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 215-244 amino acids from the Central region of human SPARCL1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SPARCL1 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the SPARCL1 antibody detected the SPARCL1 protein (arrow).)

Immunohistochemistry (IHC)

(SPARCL1 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPARCL1 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-SPARCL1 antibody

Antibodies; Proteins

References

Davila, S., et al. Genes Immun. 11(3):232-238(2010). Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009): Kahler, A.K., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (7), 1089-1100 (2008): Weimer, J.M., et al. Glia 56(9):935-941(2008). Scalabrini, D., et al. Neurosci. Lett. 425(3):173-176(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 190194423
• NCBI GeneID: 8404
• NCBI Accession #: NP_001121782.1; NP_004675.3
• NCBI GenBank Nucleotide #: NM_001128310.2
• UniProt Accession #: Q14515; Q14800; B4E2Z0; E7ESU2
• Molecular Weight: 61,760 Da
• NCBI Official Full Name: SPARC-like protein 1 isoform 1
• NCBI Official Synonym Full Names: SPARC like 1
• NCBI Official Symbol: SPARCL1
• NCBI Official Synonym Symbols: SC1; MAST9; PIG33; MAST 9
• NCBI Protein Information: SPARC-like protein 1
• UniProt Protein Name: SPARC-like protein 1
• Protein Family: SPARC-like protein
• UniProt Gene Name: SPARCL1
• UniProt Synonym Gene Names: Hevin
• UniProt Entry Name: SPRL1_HUMAN

Uniprot Description

SPARCL1: Belongs to the SPARC family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: extracellular space; proteinaceous extracellular matrix

Molecular Function: calcium ion binding

Biological Process: signal transduction