Rabbit SPAG9 Polyclonal Antibody | anti-SPAG9 antibody

SPAG9 Polyclonal Antibody

Cat. Num. AAA3006511

• Gene Names: SPAG9; JLP; CT89; HLC4; HLC6; JIP4; PHET; PIG6; HLC-6; JIP-4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SPAG9; Polyclonal Antibody; SPAG9 Polyclonal Antibody; C-Jun-amino-terminal kinase-interacting protein 4; JIP-4; JNK-interacting protein 4; Cancer/testis antigen 89; CT89; Human lung cancer oncogene 6 protein; HLC-6; JNK-associated leucine-zipper protein; JLP; Mitogen-activated protein kinase 8-interacting protein 4; Proliferation-inducing protein 6; Protein highly expressed in testis; PHET; Sperm surface protein; Sperm-associated antigen 9; Sperm-specific protein; Sunday driver 1; HSS; KIAA0516; MAPK8IP4; SYD1; HLC6; anti-SPAG9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SPAG9 polyclonal antibody detects endogenous levels of SPAG9 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1321

• Applicable Applications for anti-SPAG9 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human SPAG9.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SPAG9 pAb at 1:500 dilutionLane1:AML-12 whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:EC9706 whole cell lysate (40ug) Lane4:SGC7901 whole cell lysate (40ug))

Related Product Information for anti-SPAG9 antibody

JIP-4 (C-jun-amino-terminal kinase-interacting protein 4, Mitogen-activated protein kinase 8-interacting protein 4, Sunday driver 1) is a 1, 321 amino acid protein encoded by the human gene SPAG9. It contains a large N-terminal extracellular domain, a short transmembrane helical domain, and a cytoplasmic domain. There are 6 N-glycosylation sites, several phosphorylation sites for cAMP/cGMP-dependent protein kinase, protein kinase C, and casein kinase II, and 10 putative myristoylation sites. There is also a leucine zipper motif, with 6 leucine repeats, that may aid in dimerization since there is no upstream basic domain characteristic of DNA binding proteins. The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP-4 is a cytoplasmic, perinuclear protein that has eight known isoforms whose expression varies by tissue and disease state.

NCBI and Uniprot Product Information

• NCBI GI #: 158253415
• NCBI GeneID: 9043
• UniProt Accession #: O60271
• Molecular Weight: ~ 146kDa
• NCBI Official Full Name: SPAG9 protein
• NCBI Official Synonym Full Names: sperm associated antigen 9
• NCBI Official Symbol: SPAG9
• NCBI Official Synonym Symbols: JLP; CT89; HLC4; HLC6; JIP4; PHET; PIG6; HLC-6; JIP-4
• NCBI Protein Information: C-Jun-amino-terminal kinase-interacting protein 4
• UniProt Protein Name: C-Jun-amino-terminal kinase-interacting protein 4
• Protein Family: C-Jun-amino-terminal kinase-interacting protein
• UniProt Gene Name: SPAG9
• UniProt Synonym Gene Names: HSS; KIAA0516; MAPK8IP4; SYD1; JIP-4; JNK-interacting protein 4; CT89; HLC-6; JLP; PHET

NCBI Description

This gene encodes a member of the cancer testis antigen gene family. The encoded protein functions as a scaffold protein that structurally organizes mitogen-activated protein kinases and mediates c-Jun-terminal kinase signaling. This protein also binds to kinesin-1 and may be involved in microtubule-based membrane transport. This protein may play a role in tumor growth and development. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011]

Uniprot Description

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Isoform 5 may play a role in spermatozoa-egg-interaction.