Western Blot (WB)
(Western Blot analysis of SPAG6 expression in transfected 293T cell line by SPAG6 polyclonal antibody. Lane 1: SPAG6 transfected lysate (55.5kD). Lane 2: Non-transfected lysate.)
Rabbit anti-Human SPAG6 Polyclonal Antibody | anti-SPAG6 antibody
SPAG6 (Sperm-associated Antigen 6, Protein PF16 Homolog, PF16, Repro-SA-1, Sperm Flagellar Protein) (HRP)
Gene Names
SPAG6; pf16; CT141; FAP194; CFAP194; Repro-SA-1
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SPAG6; Polyclonal Antibody; SPAG6 (Sperm-associated Antigen 6; Protein PF16 Homolog; PF16; Repro-SA-1; Sperm Flagellar Protein) (HRP); anti-SPAG6 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human SPAG6.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Sequence Length
2630
Applicable Applications for anti-SPAG6 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human SPAG6, aa1-509 (NP_036575.1).
Immunogen Sequence
MSQRQVLQVFEQYQKARTQFVQMVAELATRPQNIETLQNAGVMSLLRTLLLDVVPTIQQTAALALGRLANYNDDLAEAVVKCDILPQLVYSLAEQNRFYKKAAAFVLRAVGKHSPQLAQAIVDCGALDTLVICLEDFDPGVKEAAAWALRYIARHNAELSQAVVDAGAVPLLVLCIQEPEIALKRIAASALSDIAKHSPELAQTVVDAGAVAHLAQMILNPDAKLKHQILSALSQVSKHSVDLAEMVVEAEIFPVVLTCLKDKDEYVKKNASTLIREIAKHTPELSQLVVNAGGVAAVIDCIGSCKGNTRLPGIMMLGYVAAHSENLAMAVIISKGVPQLSVCLSEEPEDHIKAAAAWALGQIGRHTPEHARAVAVTNTLPVLLSLYMSTESSEDLQVKSKKAIKNILQKCTYLPALEPFLYDAPPNILKHVVGQFSKVLPHDSKARRLFVTSGGLKKVQEIKAEPGSLLQEYINSINSCYPEEIVRYYSPGYSDTLLQRVDSYQPLNN
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of SPAG6 expression in transfected 293T cell line by SPAG6 polyclonal antibody. Lane 1: SPAG6 transfected lysate (55.5kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of SPAG6 expression in transfected 293T cell line by SPAG6 polyclonal antibody. Lane 1: SPAG6 transfected lysate (55.5kD). Lane 2: Non-transfected lysate.)
Product Categories/Family for anti-SPAG6 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant 1, mRNA
NCBI Official Synonym Full Names
sperm associated antigen 6
NCBI Official Symbol
SPAG6
NCBI Official Synonym Symbols
pf16; CT141; FAP194; CFAP194; Repro-SA-1
NCBI Protein Information
sperm-associated antigen 6
UniProt Protein Name
Sperm-associated antigen 6
Protein Family
UniProt Gene Name
SPAG6
UniProt Synonym Gene Names
PF16
UniProt Entry Name
SPAG6_HUMAN
NCBI Description
The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from an infertile man. This protein localizes to the tail of permeabilized human sperm and contains eight contiguous armadillo repeats, a motif known to mediate protein-protein interactions. Studies in mice suggest that this protein is involved in sperm flagellar motility and maintenance of the structural integrity of mature sperm. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
Research Articles on SPAG6
Similar Products
Product Notes
The SPAG6 spag6 (Catalog #AAA6394854) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPAG6 (Sperm-associated Antigen 6, Protein PF16 Homolog, PF16, Repro-SA-1, Sperm Flagellar Protein) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPAG6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SPAG6 spag6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPAG6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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