RNA Binding Motif Protein 20 (RBM20) Recombinant Protein | RBM20 recombinant protein

Recombinant RNA Binding Motif Protein 20 (RBM20)

Cat. Num. AAA2009112

• Reactivity: Homo sapiens (Human)
• Applications: SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: RNA Binding Motif Protein 20 (RBM20); Recombinant RNA Binding Motif Protein 20 (RBM20); RBM20 recombinant protein

• Host: E.coli
• Reactivity: Homo sapiens (Human)
• Purity/Purification: > 90%
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
• Concentration: Original Concentration: 250ug/mL (varies by lot)
• Sequence: MGHHHHHHSGSEF-PI ETDLQNQACQ EVLTPENSRY VEMKSLEVRS PEYTEVELKQ PLSLPSWEPE DVFSELSIPL GVEFVVPRTG FYCKLCGLFY TSEETAKMSH CRSAVHYRNL QKYLSQLAEE GLKETEGADS PRPEDSGIVP RFERKKL

• Applicable Applications for RBM20 recombinant protein: Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
• Application Notes: (May be suitable for use in other assays to be determined by the end user.)
• Source: Prokaryotic expression
• Residues: Pro1089~Leu1227
• Tags: N-terminal His Tag
• Subcellular Location: Nucleus
• Traits: Freeze-dried powder
• Predicted isoelectric point: 5.4
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 2-8 degree C for one month. ; Aliquot and store at -80 degree C for 12 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition

Gene Sequencing (Extract)

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 197276594
• NCBI GeneID: 282996
• NCBI Accession #: NP_001127835.1
• NCBI GenBank Nucleotide #: NM_001134363.1
• UniProt Accession #: Q5T481; Q5JVI1; A6NIP5; B5A868
• Molecular Weight: Predicted Molecular Mass: 17.4kDa; Accurate Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: RNA-binding protein 20
• NCBI Official Synonym Full Names: RNA binding motif protein 20
• NCBI Official Symbol: RBM20
• NCBI Protein Information: RNA-binding protein 20; RNA-binding motif protein 20; probable RNA-binding protein 20
• UniProt Protein Name: RNA-binding protein 20
• Protein Family: RNA-binding protein
• UniProt Gene Name: RBM20
• UniProt Entry Name: RBM20_HUMAN

NCBI Description

This gene encodes a protein that binds RNA and regulates splicing. Mutations in this gene have been associated with familial dilated cardiomyopathy. [provided by RefSeq, Apr 2014]

Uniprot Description

RBM20: RNA-binding protein that acts as a regulator of mRNA splicing of a subset of genes involved in cardiac development. Regulates splicing of TTN (Titin). Defects in RBM20 are the cause of cardiomyopathy dilated type 1DD (CMD1DD). A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

Chromosomal Location of Human Ortholog: 10q25.2

Cellular Component: nucleus

Molecular Function: zinc ion binding; RNA binding; nucleotide binding

Biological Process: heart development; RNA splicing; mRNA processing; positive regulation of RNA splicing

Disease: Cardiomyopathy, Dilated, 1dd