Goat SPAG16 Polyclonal Antibody | anti-SPAG16 antibody
Goat anti-SPAG16 (aa139-152) Antibody
Gene Names
SPAG16; PF20; WDR29
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Dog, Cow
Applications
ELISA, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
SPAG16; Polyclonal Antibody; Goat anti-SPAG16 (aa139-152) Antibody; Goat Anti-SPAG16 Antibody; sperm associated antigen 16; DKFZp666P1710; FLJ22724; FLJ37717; MGC87036; PF20; WDR29; WD repeat domain 29; sperm-associated WD repeat protein; DKFZp666P1710 antibody; FLJ22724 antibody; FLJ37717 antibody; MGC87036 antibody; PF20 antibody; sperm associated antigen 16 antibody; sperm-associated WD repeat protein antibody; WD repeat domain 29 antibody; WDR29 antibody; SPAG16 antibody; SPAG16 (aa139-152); anti-SPAG16 antibody
Host
Goat
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
KGVTELRTVGNVPD
Sequence Length
631
Applicable Applications for anti-SPAG16 antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: This antibody was successfully used in Human and Murine frozen Brani sections (PMID: 25086173).
Western Blot: Preliminary experiments gave an approx 30kDa band in Human Testis lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 70.8kDa according to NP_078808.3 and of 20.6kDa according to NP_001020607.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide.
Immunohistochemistry: This antibody was successfully used in Human and Murine frozen Brani sections (PMID: 25086173).
Western Blot: Preliminary experiments gave an approx 30kDa band in Human Testis lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 70.8kDa according to NP_078808.3 and of 20.6kDa according to NP_001020607.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide.
Immunogen
Peptide with sequence C-KGVTELRTVGNVPD, from the internal region of the protein sequence according to NP_078808.3; NP_001020607.1.
Epitope
Internal region
Note
This antibody is expected to recognize isoform 1 and 2 (NP_078808.3; NP_001020607.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,739 Da
NCBI Official Full Name
sperm-associated antigen 16 protein isoform 1
NCBI Official Synonym Full Names
sperm associated antigen 16
NCBI Official Symbol
SPAG16
NCBI Official Synonym Symbols
PF20; WDR29
NCBI Protein Information
sperm-associated antigen 16 protein
UniProt Protein Name
Sperm-associated antigen 16 protein
Protein Family
UniProt Gene Name
SPAG16
UniProt Synonym Gene Names
PF20
UniProt Entry Name
SPG16_HUMAN
NCBI Description
Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively (Zhang et al., 2007 [PubMed 17699735]).[supplied by OMIM, Jul 2008]
Uniprot Description
SPAG16: Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia. 5 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 2q34
Cellular Component: axoneme; cytoplasm; cytoskeleton; nucleus
Molecular Function: protein kinase binding
Biological Process: axoneme biogenesis; cilium biogenesis; microtubule sliding; sperm axoneme assembly
Research Articles on SPAG16
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Product Notes
The SPAG16 spag16 (Catalog #AAA421785) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SPAG16 (aa139-152) Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's SPAG16 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 16000. Immunohistochemistry: This antibody was successfully used in Human and Murine frozen Brani sections (PMID: 25086173). Western Blot: Preliminary experiments gave an approx 30kDa band in Human Testis lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 70.8kDa according to NP_078808.3 and of 20.6kDa according to NP_001020607.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the SPAG16 spag16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KGVTELRTVG NVPD. It is sometimes possible for the material contained within the vial of "SPAG16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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