Western Blot (WB)
(SPAG1 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SPAG1 antibody detected the SPAG1 protein (arrow).)
Rabbit anti-Human SPAG1 Polyclonal Antibody | anti-SPAG1 antibody
SPAG1 Antibody (N-term)
Western Blot (WB)
(SPAG1 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SPAG1 antibody detected the SPAG1 protein (arrow).)
Western Blot (WB)
(SPAG1 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SPAG1 antibody detected the SPAG1 protein (arrow).)
Immunohistochemistry (IHC)
(SPAG1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPAG1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(SPAG1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPAG1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
unexplained infertility implicates a role for these antibodies in
blocking fertilization. Improved identification and treatment of
immunologic infertility, as well as identification of proteins for
targeted contraception, are dependent on the identification and
characterization of relevant sperm antigens. The protein expressed
by this gene is recognized by anti-sperm agglutinating antibodies
from an infertile woman. Furthermore, immunization of female rats
with the recombinant human protein reduced fertility. This protein
localizes to the plasma membrane of germ cells in the testis and to
the post-acrosomal plasma membrane of mature spermatozoa.
Recombinant polypeptide binds GTP and exhibits GTPase activity.
Thus, this protein may regulate GTP signal transduction pathways
involved in spermatogenesis and fertilization. Two transcript
variants of this gene encode the same protein. [provided by
RefSeq].
Diekman, A.B., et al. Am. J. Reprod. Immunol. 37(1):111-117(1997)
Zhang, M.L., et al. Chin. Med. J. 105(12):998-1003(1992)
NCBI and Uniprot Product Information
NCBI Description
The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. Furthermore, immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombinant polypeptide binds GTP and exhibits GTPase activity. Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein. [provided by RefSeq, Jul 2008]
Uniprot Description
SPAG1: Plays a role in fertilization. Binds GTP and has GTPase activity.
Protein type: Cancer Testis Antigen (CTA); Microtubule-binding
Chromosomal Location of Human Ortholog: 8q22.2
Cellular Component: nucleoplasm; microtubule cytoskeleton; cytoplasm
Molecular Function: GTP binding; hydrolase activity
Biological Process: metabolic process; single fertilization
Disease: Ciliary Dyskinesia, Primary, 28
Research Articles on SPAG1
Similar Products
Product Notes
The SPAG1 spag1 (Catalog #AAA9202803) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 124-152. The SPAG1 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPAG1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the SPAG1 spag1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPAG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
