Rabbit anti-Human SOX2 Polyclonal Antibody | anti-SOX2 antibody

SOX2, Phosphorylated (Ser83) (Transcription Factor SOX-2) (AP)

Cat. Num. AAA6500326

• Gene Names: SOX2; ANOP3; MCOPS3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SOX2; Polyclonal Antibody; Phosphorylated (Ser83) (Transcription Factor SOX-2) (AP); anti-SOX2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SOX2 when phosphorylated at Ser83.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-SOX2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser83 of human SOX2 (KLH).
• Conjugate: AP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-Phospho-SOX2-S83 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Product Categories/Family for anti-SOX2 antibody

Antibodies; Transcription Factors

References

1. Remenyi, A., et al., Genes Dev. 17(16):2048-2059 (2003). 2. Wiebe, M.S., et al., J. Biol. Chem. 278(20):17901-17911 (2003). 3. Fantes, J., et al., Nat. Genet. 33(4):461-463 (2003). 4. Schepers, G.E., et al., Dev. Cell 3(2):167-170 (2002). 5. Kamachi, Y., et al., Trends Genet. 16(4):182-187 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 28195386
• NCBI GeneID: 6657
• NCBI Accession #: NP_003097
• NCBI GenBank Nucleotide #: NM_003106.3
• UniProt Accession #: P48431; Q14537
• Molecular Weight: 34,310 Da
• NCBI Official Full Name: transcription factor SOX-2
• NCBI Official Synonym Full Names: SRY (sex determining region Y)-box 2
• NCBI Official Symbol: SOX2
• NCBI Official Synonym Symbols: ANOP3; MCOPS3
• NCBI Protein Information: transcription factor SOX-2; transcription factor SOX2; SRY-related HMG-box gene 2
• UniProt Protein Name: Transcription factor SOX-2
• UniProt Gene Name: SOX2
• UniProt Entry Name: SOX2_HUMAN

NCBI Description

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]