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Typical Testing Data/Standard Curve (for reference only)

Human INF Kappa B (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor epsilon, Nfkbie) ELISA Kit | INF Kappa B elisa kit

Human INF Kappa B (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor epsilon, Nfkbie) ELISA Kit

Gene Names
NFKBIE; IKBE
Reactivity
Human
Synonyms
INF Kappa B (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor epsilon; Nfkbie); Human INF Kappa B (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor epsilon; Nfkbie) ELISA Kit; INF Kappa B elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Sequence Length
500
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for INF Kappa B elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- INF Kappa B antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-INF Kappa B antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the INF Kappa B amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of INF Kappa B can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
52,864 Da
NCBI Official Full Name
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon, isoform CRA_a
NCBI Official Synonym Full Names
NFKB inhibitor epsilon
NCBI Official Symbol
NFKBIE
NCBI Official Synonym Symbols
IKBE
NCBI Protein Information
NF-kappa-B inhibitor epsilon
UniProt Protein Name
NF-kappa-B inhibitor epsilon
UniProt Gene Name
NFKBIE
UniProt Synonym Gene Names
IKBE; NF-kappa-BIE; IkB-E; IkB-epsilon; IkappaBepsilon

NCBI Description

The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. [provided by RefSeq, Sep 2011]

Uniprot Description

IkB-epsilon: Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes. Interacts with RELA, REL, NFKB1 nuclear factor NF-kappa-B p50 subunit and NFKB2 nuclear factor NF-kappa-B p52 subunit. Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages. Belongs to the NF-kappa-B inhibitor family.

Protein type: DNA-binding; Inhibitor

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: cytoplasm; cytosol; fibrillar center; nucleus

Molecular Function: protein binding

Biological Process: cytoplasmic sequestering of transcription factor

Research Articles on INF Kappa B

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Product Notes

The Human INF Kappa B nfkbie (Catalog #AAA7606230) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7606230 ELISA Kit recognizes Human INF Kappa B. It is sometimes possible for the material contained within the vial of "INF Kappa B (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor epsilon, Nfkbie), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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