Immunohistochemistry (IHC)
(At 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Rabbit Sodium Potassium ATPase alpha 1 Polyclonal Antibody | anti-ATP1A1 antibody
Phospho-Sodium Potassium ATPase alpha 1 (Tyr542) Antibody
IHC: 1:50-1:200
ELISA (Peptide): 1:20000-1:40000
Immunohistochemistry (IHC)
(At 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Immunohistochemistry (IHC)
(At 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Western Blot (WB)
(Western blot analysis of extracts of Rat brain tissue sample,using Phospho-ATP1 alpha1/Na+K+ ATPase1 (Tyr542) Antibody.)
Western Blot (WB)
(Western blot analysis of extracts of Rat brain tissue sample,using Phospho-ATP1 alpha1/Na+K+ ATPase1 (Tyr542) Antibody.)
Post Translational Modifications: Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity (By similarity).
Subunit Structure: The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit. Interacts with regulatory subunit FXYD1 (By similarity). Interacts with regulatory subunit FXYD3 (PubMed:21454534). Interacts with SIK1 (By similarity). Binds the HLA class II histocompatibility antigen DR1 (PubMed:1380674). Interacts with SLC35G1 and STIM1 (PubMed:22084111).
Similarity: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
NCBI and Uniprot Product Information
Predicted: 113 kDa
NCBI Description
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
Uniprot Description
ATP1A1: the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na and K ions across the plasma membrane. This action creates the electrochemical gradient of Na and K, providing the energy for active transport of various nutrients. Two splice-variant isoforms have been described.
Protein type: Membrane protein, integral; Transporter, ion channel; EC 3.6.3.9; Membrane protein, multi-pass; Hydrolase; Transporter
Chromosomal Location of Human Ortholog: 1p21
Cellular Component: Golgi apparatus; protein complex; basolateral plasma membrane; endoplasmic reticulum; T-tubule; integral to membrane; caveola; membrane; apical plasma membrane; plasma membrane; melanosome; sodium:potassium-exchanging ATPase complex; sarcolemma; endosome; vesicle
Molecular Function: potassium ion binding; protein domain specific binding; protein binding; sodium ion binding; chaperone binding; phosphoric monoester hydrolase activity; sodium:potassium-exchanging ATPase activity; phosphoinositide 3-kinase binding; ankyrin binding; ADP binding; protein kinase binding; ATP binding
Biological Process: response to drug; cellular sodium ion homeostasis; negative regulation of heart contraction; membrane hyperpolarization; positive regulation of striated muscle contraction; regulation of sodium ion transport; cellular potassium ion homeostasis; negative regulation of glucocorticoid biosynthetic process; dephosphorylation; potassium ion import; regulation of blood pressure; positive regulation of heart contraction; transmembrane transport; regulation of the force of heart contraction; cardiac muscle contraction
Research Articles on ATP1A1
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Product Notes
The ATP1A1 atp1a1 (Catalog #AAA9610563) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Sodium Potassium ATPase alpha 1 (Tyr542) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Sodium Potassium ATPase alpha 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA (Peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the ATP1A1 atp1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sodium Potassium ATPase alpha 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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