Rabbit TCF7 Polyclonal Antibody | anti-TCF7 antibody

TCF7 (Transcription Factor 7, TCF-1) (Biotin)

Cat. Num. AAA6440149

• Gene Names: TCF7; TCF-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: TCF7; Polyclonal Antibody; TCF7 (Transcription Factor 7; TCF-1) (Biotin); anti-TCF7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes TCF7. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-TCF7 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to human TCF7.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using TCF7 antibody.)

Product Categories/Family for anti-TCF7 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 293651582
• NCBI GeneID: 6932
• NCBI Accession #: NP_001128323.2
• NCBI GenBank Nucleotide #: NM_001134851.2
• UniProt Accession #: P36402; Q86WR9; Q9UKI4; B3KSH3
• Molecular Weight: 41,642 Da
• NCBI Official Full Name: transcription factor 7 isoform 3
• NCBI Official Synonym Full Names: transcription factor 7 (T-cell specific, HMG-box)
• NCBI Official Symbol: TCF7
• NCBI Official Synonym Symbols: TCF-1
• NCBI Protein Information: transcription factor 7; T-cell-specific transcription factor 1
• UniProt Protein Name: Transcription factor 7
• Protein Family: Transcription factor
• UniProt Gene Name: TCF7
• UniProt Synonym Gene Names: TCF1; TCF-7; T-cell factor 1; TCF-1
• UniProt Entry Name: TCF7_HUMAN

NCBI Description

The protein encoded by this gene is a transcriptional activator that plays an important role in lymphocyte differentiation. This gene is expressed predominantly in T-cells. The encoded protein can bind an enhancer element and activate the CD3E gene, and it also may repress the CTNNB1 and TCF7L2 genes through a feedback mechanism. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

TCF7: Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T- lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1. Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with AES, TLE1, TLE2, TLE3 and TLE4. By TCF7L2 and CTNNB1. Predominantly expressed in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium. Belongs to the TCF/LEF family. 16 isoforms of the human protein are produced by alternative promoter.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: nucleoplasm; transcription factor complex; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; beta-catenin binding; chromatin binding; transcription factor activity

Biological Process: neural tube development; transcription, DNA-dependent; alpha-beta T cell differentiation; Wnt receptor signaling pathway through beta-catenin; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; embryonic digestive tract morphogenesis; generation of neurons; T cell receptor V(D)J recombination; immune response; brain development; embryonic genitalia morphogenesis; embryonic hindgut morphogenesis