Rabbit Sodium Potassium ATPase Monoclonal Antibody | anti-ATP1A1 antibody

Sodium Potassium ATPase Antibody

Cat. Num. AAA9435718

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: Sodium Potassium ATPase; Monoclonal Antibody; Sodium Potassium ATPase Antibody; ATPase Na+/K+ transporting alpha antibody; adenosinetriphosphatase antibody; AT1A1_HUMAN antibody; ATP1A1 antibody; ATP1A4 antibody; ATP1AL2 antibody; ATP1B antibody; ATP1B1 antibody; ATPase Na+/K+ transporting alpha 1 polypeptide antibody; ATPase Na+/K+ transporting alpha 4 polypeptide antibody; ATPase Na+/K+ transporting beta 1 polypeptide antibody; ATPase; Na+/K+ transporting; alpha polypeptide-like 2 antibody; beta 1 polypeptide antibody; Beta 1-subunit of Na (+); K (+)-ATPase antibody; Na (+)/K (+) ATPase alpha-1 subunit antibody; Na (+)/K (+) ATPase alpha-4 subunit antibody; Na+; K+ ATPase alpha subunit antibody; Na+/K+ ATPase 1 antibody; Na+/K+ ATPase 4 antibody; Na+/K+ ATPase; alpha-D polypeptide antibody; Na; K-ATPase beta-1 polypeptide antibody; K-ATPase; alpha-A catalytic polypeptide antibody; K-ATPase catalytic subunit alpha-A protein antibody; K-ATPase subunit alpha-C antibody; polypeptide-like 2 antibody; Sodium pump 1 antibody; sodium pump 4 antibody; Sodium pump subunit alpha-1 antibody; sodium pump subunit alpha-4 antibody; sodium pump subunit beta-1 antibody; sodium-potassium ATPase catalytic subunit alpha-1 antibody; sodium-potassium ATPase catalytic subunit alpha-4 antibody; sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody; sodium-potassium ATPase; alpha 4 polypeptide antibody; sodium-potassium-ATPase; alpha 1 polypeptide antibody; Sodium/potassium transporting ATPase alpha 1 chain antibody; Sodium/potassium transporting ATPase subunit beta 1 antibody; sodium/potassium-dependent ATPase beta-1 subunit antibody; sodium/potassium-transporting ATPase alpha-4 chain antibody; sodium/potassium-transporting ATPase beta-1 chain antibody; Sodium/potassium-transporting ATPase subunit alpha-1 antibody; sodium/potassium-transporting ATPase subunit alpha-4 antibody; anti-ATP1A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone Number: ST0533
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-ATP1A1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-5000; IHC: 1:50-1:200; ICC: 1:50-1:200; FC/FACS: 1:50-1:100
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant protein
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Sodium Potassium ATPase on A549 cell lysates using anti-Sodium Potassium ATPase antibody at 1/1, 000 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining Sodium Potassium ATPase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining Sodium Potassium ATPase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Hela cells with Sodium Potassium ATPase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

Related Product Information for anti-ATP1A1 antibody

The ubiquitously expressed sodium/potassium-ATPase (Na+/K+-ATPase) exists as an oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na+ ions and two K+ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na+/K+-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, alpha, beta and gamma, comprise the Na+/K+-ATPase oligomer. The alpha subunit contains the binding sites for ATP and the cations; the glycosylated beta subunit ensures correct folding and membrane insertion of the alpha subunits. The small gamma subunit co-localizes with the alpha subunit in nephron segments, where it increases the affinity of Na+/K+-ATPase for ATP. The beta subunit, but not the gamma subunit, is essential for normal activity of Na+/K+-ATPase.

Product Categories/Family for anti-ATP1A1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 21361181
• NCBI GeneID: 476
• NCBI Accession #: NP_000692.2
• NCBI GenBank Nucleotide #: NM_000701.7
• UniProt Accession #: P05023; Q16689; Q6LDM4; Q9UCN1; Q9UJ20; Q9UJ21; B2RBR6; B7Z3U6
• Molecular Weight: 112,896 Da
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit alpha-1 isoform a
• NCBI Official Synonym Full Names: ATPase, Na+/K+ transporting, alpha 1 polypeptide
• NCBI Official Symbol: ATP1A1
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit alpha-1; sodium pump 1; Na+/K+ ATPase 1; Na,K-ATPase alpha-1 subunit; sodium pump subunit alpha-1; Na+, K+ ATPase alpha subunit; Na(+)/K(+) ATPase alpha-1 subunit; Na, K-ATPase, alpha-A catalytic polypeptide; s
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit alpha-1
• Protein Family: Sodium/potassium-transporting ATPase
• UniProt Gene Name: ATP1A1
• UniProt Synonym Gene Names: Na(+)/K(+) ATPase alpha-1 subunit
• UniProt Entry Name: AT1A1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

ATP1A1: the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na and K ions across the plasma membrane. This action creates the electrochemical gradient of Na and K, providing the energy for active transport of various nutrients. Two splice-variant isoforms have been described.

Protein type: Membrane protein, integral; Transporter, ion channel; EC 3.6.3.9; Membrane protein, multi-pass; Hydrolase; Transporter

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: Golgi apparatus; protein complex; basolateral plasma membrane; endoplasmic reticulum; T-tubule; integral to membrane; caveola; membrane; apical plasma membrane; plasma membrane; melanosome; sodium:potassium-exchanging ATPase complex; sarcolemma; endosome; vesicle

Molecular Function: potassium ion binding; protein domain specific binding; protein binding; sodium ion binding; chaperone binding; phosphoric monoester hydrolase activity; sodium:potassium-exchanging ATPase activity; phosphoinositide 3-kinase binding; ankyrin binding; ADP binding; protein kinase binding; ATP binding

Biological Process: response to drug; cellular sodium ion homeostasis; negative regulation of heart contraction; membrane hyperpolarization; positive regulation of striated muscle contraction; regulation of sodium ion transport; cellular potassium ion homeostasis; negative regulation of glucocorticoid biosynthetic process; dephosphorylation; potassium ion import; regulation of blood pressure; positive regulation of heart contraction; transmembrane transport; regulation of the force of heart contraction; cardiac muscle contraction