Goat SNX26/TCGAP Polyclonal Antibody | anti-SNX26 antibody

Goat anti-SNX26/TCGAP (N Terminus) Antibody

Cat. Num. AAA420263

• Gene Names: ARHGAP33; SNX26; TCGAP; NOMA-GAP
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: SNX26/TCGAP; Polyclonal Antibody; Goat anti-SNX26/TCGAP (N Terminus) Antibody; Goat Anti-SNX26 / TCGAP (C Terminus) Antibody; SNX26; TCGAP; sorting nexin 26; FLJ39019; SNX26 antibody; TCGAP antibody; sorting nexin 26 antibody; FLJ39019 antibody; SNX26 / TCGAP (N Terminus); anti-SNX26 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: ARSTDSLDGPGE
• Sequence Length: 1126

• Applicable Applications for anti-SNX26 antibody: Peptide ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Immunohistochemistry: In paraffin embedded Human Prostate shows cytoplasm staining of secretory cells in the glands. Recommended concentration, 1.25ug/ml.; Western Blot: Preliminary experiments gave an approx 35kDa band in human brain (hippocampus) lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 121kDa according to NP_443180.2. The 35kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence ARSTDSLDGPGE-C, from the N Terminus of the protein sequence according to NP_443180.2.
• Epitope: N Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(1.25ug/ml) staining of paraffin embedded human prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 23510413
• NCBI GeneID: 115703
• NCBI Accession #: NP_443180.2
• NCBI GenBank Nucleotide #: NM_052948.3
• Molecular Weight: 26,858 Da
• NCBI Official Full Name: rho GTPase-activating protein 33 isoform 1
• NCBI Official Synonym Full Names: Rho GTPase activating protein 33
• NCBI Official Symbol: ARHGAP33
• NCBI Official Synonym Symbols: SNX26; TCGAP; NOMA-GAP
• NCBI Protein Information: rho GTPase-activating protein 33
• UniProt Protein Name: Rho GTPase-activating protein 33
• UniProt Gene Name: ARHGAP33
• UniProt Synonym Gene Names: SNX26; TCGAP
• UniProt Entry Name: RHG33_HUMAN

NCBI Description

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. Alternative splice variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Feb 2010]

Uniprot Description

SNX26: May be involved in several stages of intracellular trafficking. Could play an important role in the regulation of glucose transport by insulin. May act as a downstream effector of RHOQ/TC10 in the regulation of insulin-stimulated glucose transport. Belongs to the PX domain-containing GAP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; GAPs, Rac/Rho; GAPs

Chromosomal Location of Human Ortholog: 19q13.12

Cellular Component: cytosol

Molecular Function: GTPase activator activity; phosphoinositide binding; protein binding

Biological Process: positive regulation of GTPase activity; protein transport; regulation of small GTPase mediated signal transduction; signal transduction