Immunohistochemistry (IHC)
(5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Goat THBS3 Polyclonal Antibody | anti-THBS3 antibody
Goat anti-THBS3 Antibody
Gene Names
THBS3; TSP3
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Dog, Cow, Pig
Applications
ELISA, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
THBS3; Polyclonal Antibody; Goat anti-THBS3 Antibody; thrombospondin 3; MGC119564; MGC119565; TSP3; OTTHUMP00000033827; THBS3 antibody; thrombospondin 3 antibody; MGC119564 antibody; MGC119565 antibody; TSP3 antibody; OTTHUMP00000033827 antibody; anti-THBS3 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Dog, Cow, Pig
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence
CNDTVPEDFEPFRRQ
Applicable Applications for anti-THBS3 antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Colon shows endothelial cell staining of a blood vessel. Recommended concentration, 1-2ug/ml.
Western Blot: Preliminary experiments gave an approx 50kDa band in Human Lung and in lysates of cell line A549 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104kDa according to NP_009043.1. The 50kDa band was successfully blocked by incubation with the immunizing peptide.
Immunohistochemistry: In paraffin embedded Human Colon shows endothelial cell staining of a blood vessel. Recommended concentration, 1-2ug/ml.
Western Blot: Preliminary experiments gave an approx 50kDa band in Human Lung and in lysates of cell line A549 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104kDa according to NP_009043.1. The 50kDa band was successfully blocked by incubation with the immunizing peptide.
Immunogen
Peptide with sequence CNDTVPEDFEPFRRQ, from the C Terminus of the protein sequence according to NP_009043.1.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Immunohistochemistry (IHC)
(5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
(5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
91,297 Da
NCBI Official Full Name
thrombospondin-3 isoform 1
NCBI Official Synonym Full Names
thrombospondin 3
NCBI Official Symbol
THBS3
NCBI Official Synonym Symbols
TSP3
NCBI Protein Information
thrombospondin-3
UniProt Protein Name
Thrombospondin-3
Protein Family
UniProt Gene Name
THBS3
UniProt Synonym Gene Names
TSP3
UniProt Entry Name
TSP3_HUMAN
NCBI Description
The protein encoded by this gene belongs to the thrombospondin family. Thrombospondin family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentameric molecule linked by a single disulfide bond. This gene shares a common promoter with metaxin 1. Alternate splicing results in coding and non-coding transcript variants. [provided by RefSeq, Nov 2011]
Uniprot Description
THBS3: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen. Belongs to the thrombospondin family.
Protein type: Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: extracellular region; perinuclear region of cytoplasm
Molecular Function: calcium ion binding; heparin binding
Biological Process: cell-matrix adhesion
Research Articles on THBS3
Similar Products
Product Notes
The THBS3 thbs3 (Catalog #AAA422053) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-THBS3 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Dog, Cow, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's THBS3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Colon shows endothelial cell staining of a blood vessel. Recommended concentration, 1-2ug/ml. Western Blot: Preliminary experiments gave an approx 50kDa band in Human Lung and in lysates of cell line A549 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104kDa according to NP_009043.1. The 50kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the THBS3 thbs3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CNDTVPEDFE PFRRQ. It is sometimes possible for the material contained within the vial of "THBS3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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