Rabbit SNRPD1 Polyclonal Antibody | anti-SNRPD1 antibody

SNRPD1 Antibody

Cat. Num. AAA9630805

• Gene Names: SNRPD1; SMD1; SNRPD; Sm-D1; HsT2456
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: SNRPD1; Polyclonal Antibody; SNRPD1 Antibody; AA407109; AL023031; HsT2456; Sm D1; Sm-D autoantigen; Sm-D1; Small nuclear ribonucleoprotein D1 polypeptide; Small nuclear ribonucleoprotein Sm D1; SmD autoantigen; SMD1; SMD1_HUMAN; snRNP core protein D1; SNRPD; Snrpd1; anti-SNRPD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: SNRPD1 Antibody detects endogenous levels of SNRPD1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-SNRPD1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human SNRPD1.
• Conjugation: Unconjugated
• Similarity: Belongs to the snRNP core protein family.
• Post Translational Modifications: Methylated on arginine residues by PRMT5 and PRMT7; probable asymmetric dimethylation which is required for assembly and biogenesis of snRNPs.
• Subunit Structure: Core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Most spliceosomal snRNPs contain a common set of Sm proteins, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. Component of the U1 snRNP. The U1 snRNP is composed of the U1 snRNA and the 7 core Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG, and at least three U1 snRNP-specific proteins SNRNP70/U1-70K, SNRPA/U1-A and SNRPC/U1-C. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Part of the SMN-Sm complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8, STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG; catalyzes core snRNPs assembly. Forms a 6S pICln-Sm complex composed of CLNS1A/pICln, SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG; ring-like structure where CLNS1A/pICln mimics additional Sm proteins and which is unable to assemble into the core snRNP.
• Function: Plays role in pre-mRNA splicing as core component of the SMN-Sm complex that mediates spliceosomal snRNP assembly and as component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes. Is also a component of the minor U12 spliceosome. May act as a charged protein scaffold to promote snRNP assembly or strengthen snRNP-snRNP interactions through non-specific electrostatic contacts with RNA (Probable).
• Note: MN-mediated assembly into core snRNPs occurs in the cytosol before SMN-mediated transport to the nucleus to be included in spliceosomes.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-SNRPD1 antibody

Cytoplasm; Cytosol; Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 119621535
• NCBI GeneID: 6632
• UniProt Accession #: P62314; P13641; B5BTZ1
• Molecular Weight: 13,282 Da
• NCBI Official Full Name: small nuclear ribonucleoprotein D1 polypeptide 16kDa
• NCBI Official Synonym Full Names: small nuclear ribonucleoprotein D1 polypeptide 16kDa
• NCBI Official Symbol: SNRPD1
• NCBI Official Synonym Symbols: SMD1; SNRPD; Sm-D1; HsT2456
• NCBI Protein Information: small nuclear ribonucleoprotein Sm D1; Sm-D autoantigen; snRNP core protein D1; small nuclear ribonucleoprotein D1 polypeptide 16kDa pseudogene
• UniProt Protein Name: Small nuclear ribonucleoprotein Sm D1
• Protein Family: Small nuclear ribonucleoprotein
• UniProt Gene Name: SNRPD1
• UniProt Synonym Gene Names: Sm-D1
• UniProt Entry Name: SMD1_HUMAN

NCBI Description

This gene encodes a small nuclear ribonucleoprotein that belongs to the SNRNP core protein family. The protein may act as a charged protein scaffold to promote SNRNP assembly or strengthen SNRNP-SNRNP interactions through nonspecific electrostatic contacts with RNA. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]

Uniprot Description

snRNP D1: May act as a charged protein scaffold to promote snRNP assembly or strengthen snRNP-snRNP interactions through nonspecific electrostatic contacts with RNA. Belongs to the snRNP core protein family.

Protein type: Spliceosome; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: small nuclear ribonucleoprotein complex; nucleoplasm; snRNP U1; cytosol; nucleus; U12-dependent spliceosome

Molecular Function: protein binding; RNA binding

Biological Process: nuclear mRNA splicing, via spliceosome; spliceosomal snRNP biogenesis; RNA splicing; spliceosome assembly; gene expression