Rabbit anti-Human SNRPA Polyclonal Antibody | anti-SNRPA antibody

SNRPA, ID (SNRPA, U1 small nuclear ribonucleoprotein A) (Biotin)

Cat. Num. AAA6349399

• Gene Names: SNRPA; U1A; Mud1; U1-A
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SNRPA; Polyclonal Antibody; ID (SNRPA; U1 small nuclear ribonucleoprotein A) (Biotin); U1 small nuclear ribonucleoprotein A; anti-SNRPA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-SNRPA antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SNRPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-111 amino acids from the Central region of human SNRPA.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SNRPA Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the SNRPA antibody detected the SNRPA protein (arrow).)

Immunohistochemistry (IHC)

(SNRPA Antibody (Center) (AP14764c)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SNRPA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-SNRPA antibody

The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation.

Product Categories/Family for anti-SNRPA antibody

Antibodies; Nuclear Proteins

References

Guan, F., et al. RNA 13(12):2129-2140(2007). Hall-Pogar, T., et al. RNA 13(7):1103-1115(2007). Shajani, Z., et al. J. Mol. Biol. 349(4):699-715(2005). Andersen, J.S., et al. Nature 433(7021):77-83(2005). Jessen, T.H., et al. EMBO J. 10(11):3447-3456(1991).

NCBI and Uniprot Product Information

• NCBI GI #: 4759156
• NCBI GeneID: 6626
• NCBI Accession #: NP_004587.1
• NCBI GenBank Nucleotide #: NM_004596.4
• UniProt Accession #: P09012
• Molecular Weight: 31,280 Da
• NCBI Official Full Name: U1 small nuclear ribonucleoprotein A
• NCBI Official Synonym Full Names: small nuclear ribonucleoprotein polypeptide A
• NCBI Official Symbol: SNRPA
• NCBI Official Synonym Symbols: U1A; Mud1; U1-A
• NCBI Protein Information: U1 small nuclear ribonucleoprotein A; U1 snRNP A; U1 snRNP-specific protein A; U1 small nuclear RNP-specific A
• UniProt Protein Name: U1 small nuclear ribonucleoprotein A
• Protein Family: U1 small nuclear ribonucleoprotein
• UniProt Gene Name: SNRPA
• UniProt Synonym Gene Names: U1 snRNP A; U1-A; U1A
• UniProt Entry Name: SNRPA_HUMAN

NCBI Description

The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation. [provided by RefSeq, Oct 2010]

Uniprot Description

Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. SNRPA binds stem loop II of U1 snRNA. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. May bind preferentially to the 5'-UGCAC-3' motif on RNAs.