Rabbit SnoN Polyclonal Antibody | anti-SnoN antibody

SnoN Antibody

Cat. Num. AAA9411542

• Gene Names: SKIL; SNO; SnoA; SnoI; SnoN
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: SnoN; Polyclonal Antibody; SnoN Antibody; Ski-like protein; oncogene Sno; anti-SnoN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 684

• Applicable Applications for anti-SnoN antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 16 amino acid peptide from near the amino terminus of human SnoN.
• Target Name: SnoN
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of SnoN in A431 cell lysate with SnoN antibody at (A) 0.5 and (B) 1 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of SnoN in mouse lung tissue with SnoN antibody at 5 ug/mL.)

Related Product Information for anti-SnoN antibody

TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Like Ski, SnoN functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. SnoN is located primarily in the nucleus in cancer tissues or cells, but in the cytoplasm in normal tissues or primary epithelial cells. There are at least four alternately spliced isoforms of SnoN; SnoN antibody will recognize all isoforms (SnoN, SnoN2, SnoI, and SnoA).

Product Categories/Family for anti-SnoN antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 223029418
• NCBI GeneID: 6498
• NCBI Accession #: NP_005405
• NCBI GenBank Nucleotide #: NM_005414.4
• Molecular Weight: 74,784 Da
• NCBI Official Full Name: ski-like protein isoform 1
• NCBI Official Synonym Full Names: SKI-like proto-oncogene
• NCBI Official Symbol: SKIL
• NCBI Official Synonym Symbols: SNO; SnoA; SnoI; SnoN
• NCBI Protein Information: ski-like protein
• UniProt Protein Name: Ski-like protein
• UniProt Gene Name: SKIL
• UniProt Synonym Gene Names: SNO
• UniProt Entry Name: SKIL_HUMAN

NCBI Description

The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

SKIL: May have regulatory role in cell division or differentiation in response to extracellular signals. Interacts with SMAD2, SMAD3 and RNF111. Isoform 1 interacts with WWP1. Isoform SNON and isoform SNOA are widely expressed. Highest expression is found in skeletal muscle, followed by placenta and lung. Lowest expression in heart, brain and pancreas. Isoform SNOI expression is restricted to skeletal muscle. Belongs to the SKI family. 5 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 3q26

Cellular Component: nucleoplasm; PML body; protein complex; cytoplasm; acrosome; nucleus

Molecular Function: protein domain specific binding; protein binding; DNA binding; protein complex binding; chromatin binding; SMAD binding; transcription corepressor activity

Biological Process: transcription initiation from RNA polymerase II promoter; skeletal muscle development; transcription, DNA-dependent; blastocyst formation; negative regulation of transcription from RNA polymerase II promoter; regulation of apoptosis; response to antibiotic; negative regulation of cell differentiation; transforming growth factor beta receptor signaling pathway; response to cytokine stimulus; spermatogenesis; gene expression; positive regulation of axonogenesis; cell cycle arrest; negative regulation of transforming growth factor beta receptor signaling pathway; lymphocyte homeostasis