Rabbit anti-Human Ski-like Protein Polyclonal Antibody | anti-SKIL antibody

Ski-like Protein (Ski-related Oncogene, Ski-related Protein, SNO, SKIL)

Cat. Num. AAA647606

• Gene Names: SKIL; SNO; SnoA; SnoI; SnoN
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Ski-like Protein; Polyclonal Antibody; Ski-like Protein (Ski-related Oncogene; Ski-related Protein; SNO; SKIL); Anti -Ski-like Protein (Ski-related Oncogene; anti-SKIL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SKIL. Species sequence homology: mouse and rat
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

• Applicable Applications for anti-SKIL antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1-3ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to aa600-684 of human snoN (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Lung, Respiratory Epithelium (formalin-fixed, paraffin-embedded) stained with SKIL antibody at 5ug/ml followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-SKIL antibody

SnoN, an 80kD protein, is a member of ski family of nuclear proto-oncogenes involved in regulation of cellular transformation and differentiation. Primarily expressed in two isoforms, SnoN is localized in cytoplasm in normal tissues and non-tumorigenic primary epithelial cells. In cancer tissues or cells, SnoN is exclusively localized in the nucleus. It plays a vital role in inhibition of cell cycle arrest induced by TGF-â. Upon morphological differentiation or cell-cycle arrest, SnoN translocates into the nucleus, binds to Smad2, Smad3, and Smad4 on TGF- -responsive promoters and represses their ability to activate expression of TGF- target genes. It has also been found to mediate transcriptional repression of thyroid hormone receptor, Mad and pRb. Increased expression of SnoN has been detected in many human tumor cell lines suggesting a clinical significance.

Product Categories/Family for anti-SKIL antibody

Antibodies; Abs to Oncoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 223029422
• NCBI GeneID: 6498
• NCBI Accession #: NP_001138570.1
• NCBI GenBank Nucleotide #: NM_001145098.2
• UniProt Accession #: P12757; O00464; P12756; Q07501; A6NGT1; B4DT50
• Molecular Weight: 76,976 Da
• NCBI Official Full Name: ski-like protein isoform 3
• NCBI Official Synonym Full Names: SKI-like oncogene
• NCBI Official Symbol: SKIL
• NCBI Official Synonym Symbols: SNO; SnoA; SnoI; SnoN
• NCBI Protein Information: ski-like protein; ski-related oncogene snoN
• UniProt Protein Name: Ski-like protein
• Protein Family: Ski-like protein
• UniProt Gene Name: SKIL
• UniProt Synonym Gene Names: SNO
• UniProt Entry Name: SKIL_HUMAN

NCBI Description

The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

Function: May have regulatory role in cell division or differentiation in response to extracellular signals.

Subunit structure: Interacts with SMAD2, SMAD3 and RNF111. Isoform 1 interacts with WWP1. Ref.7 Ref.8

Tissue specificity: Isoform SNON and isoform SNOA are widely expressed. Highest expression is found in skeletal muscle, followed by placenta and lung. Lowest expression in heart, brain and pancreas. Isoform SNOI expression is restricted to skeletal muscle. Ref.6

Sequence similarities: Belongs to the SKI family.