Rabbit Ski-Like Oncogene Polyclonal Antibody | anti-SKIL antibody

Ski-Like Oncogene (SKIL, SNO, SNOA, SNON)

Cat. Num. AAA614150

• Gene Names: SKIL; SNO; SnoA; SnoI; SnoN
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Synonyms: Ski-Like Oncogene; Polyclonal Antibody; Ski-Like Oncogene (SKIL; SNO; SNOA; SNON); Anti -Ski-Like Oncogene (SKIL; anti-SKIL antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes Human Ski-like Oncogene (SKIL). Species Crossreactivity: Human, Mouse, Rat.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-SKIL antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunohistochemistry.; Dilution: Western Blot: 1:1000-1:2000; Immunohistochemistry: 2-2.5ug/ml
• Immunogen: Synthetic peptide, KLH-conjugated. Epitope: 16 amino acid peptide from near the amino terminus of human SnoN
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(SKIL (5ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human lung, respiratory epithelium, followed by biotinylated goat rabbit IgG secondary, alkaline phosphatase-streptavidin and chromogen.)

Immunohistochemistry (IHC)

(SKIL (5ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human placenta, followed by biotinylated goat rabbit IgG secondary, alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-SKIL antibody

Three Smad transcriptional co-repressors, namely Ski (Sloan-Kettering Institute proto-oncogene), SnoN (Ski-related novel gene, non Alu-containing), and TGIF (TG-interacting factor), have been identified (25,27-29). They exert transcriptional repression by numerous mechanisms (30-33). Clearly, Smad transcriptional co-repressors are unique regulatory components within the nuclei during the final stage of TGF-1 signaling; hence, their abundance and activity allow the cell to make the decision whether to proceed with the transcription of TGF-1-responsive genes. However, despite the obvious importance of Smad transcriptional co-repressors in controlling TGF-1 signaling, the regulation and relative significance of their expression in fibrotic kidney are completely unknown.

SKI is an 80-90 kD member of the SKI family of oncoproteins. SKI is best known for inhibiting TGF- signaling through its association with the SMAD proteins. Human SKI is 728 aa long and contains an N-terminal Cys-rich region (aa 94-192) and a C-terminal coiled-coil oligomerization domain (aa 536-710). Over the region used as immunogen, human and mouse SKI protein sequences are 96% identical.
The Ski and Sno proteins are components of a macromolecular complex containing the co-repressor N-CoR/SMRT, mSin3 and histone deacetylase. The complex mediates repression by a number of nuclear hormone receptors and Mad, and MeCP2. In addition, Ski and Sno can render cells resistant to TGF-beta by binding to Smads.
Ski (v-ski) was discovered as a viral oncogene from the genome of avian Sloan-Kettering retrovirus. Overexpression of both v-ski and cellular homolog c-ski in avian fibroblasts leads to anchorage-independent growth and morphological transformation of chicken embryo fibroblasts. Overexpression of c-ski has also been observed in several types of human cancer cells. New results show that Ski associates with Smads2, 3 and 4 proteins in response to TGF- stimulation. Interactions between Ski and Smad2, 3 and 4 blocks Smads-mediated transcriptional activation as well as TGF- -induced growth suppression of epithelial cells, suggesting oncogenic effects of Ski derive in part through its ability to antagonize the growth-inhibitory signals initiated by members of the TGF- family of ligands. Ski is also required for transcriptional repression mediated by Mad involved in negative regulation of cellular proliferation and plays a role in muscle differentiation.Ski (v-ski) was discovered as a viral oncogene from the genome of avian Sloan-Kettering retrovirus. Overexpression of both v-ski and cellular homolog c-ski in avian fibroblasts leads to anchorage-independent growth and morphological transformation of chicken embryo fibroblasts. Overexpression of c-ski has also been observed in several types of human cancer cells. New results show that Ski associates with Smads2, 3 and 4 proteins in response to TGF- stimulation. Interactions between Ski and Smad2, 3 and 4 blocks Smads-mediated transcriptional activation as well as TGF- -induced growth suppression of epithelial cells, suggesting oncogenic effects of Ski derive in part through its ability to antagonize the growth-inhibitory signals initiated by members of the TGF- family of ligands. Ski is also required for transcriptional repression mediated by Mad involved in negative regulation of cellular proliferation and plays a role in muscle differentiation.

Product Categories/Family for anti-SKIL antibody

Antibodies; Abs to Oncoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 223029422
• NCBI GeneID: 6498
• NCBI Accession #: NP_001138570.1
• NCBI GenBank Nucleotide #: NM_001145098.1
• UniProt Accession #: P12757; O00464; P12756; Q07501; A6NGT1; B4DT50
• Molecular Weight: 76,976 Da
• NCBI Official Full Name: ski-like protein isoform 3
• NCBI Official Synonym Full Names: SKI-like oncogene
• NCBI Official Symbol: SKIL
• NCBI Official Synonym Symbols: SNO; SnoA; SnoI; SnoN
• NCBI Protein Information: ski-like protein; OTTHUMP00000213557; OTTHUMP00000213591; OTTHUMP00000213592; OTTHUMP00000213595; ski-related protein; ski-related oncogene snoN
• UniProt Protein Name: Ski-like protein
• Protein Family: Ski-like protein
• UniProt Gene Name: SKIL
• UniProt Synonym Gene Names: SNO
• UniProt Entry Name: SKIL_HUMAN

NCBI Description

The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

SKIL: May have regulatory role in cell division or differentiation in response to extracellular signals. Interacts with SMAD2, SMAD3 and RNF111. Isoform 1 interacts with WWP1. Isoform SNON and isoform SNOA are widely expressed. Highest expression is found in skeletal muscle, followed by placenta and lung. Lowest expression in heart, brain and pancreas. Isoform SNOI expression is restricted to skeletal muscle. Belongs to the SKI family. 5 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 3q26

Cellular Component: nucleoplasm; PML body; protein complex; cytoplasm; acrosome; nucleus

Molecular Function: protein domain specific binding; protein binding; DNA binding; protein complex binding; chromatin binding; SMAD binding; transcription corepressor activity

Biological Process: transcription initiation from RNA polymerase II promoter; skeletal muscle development; transcription, DNA-dependent; blastocyst formation; negative regulation of transcription from RNA polymerase II promoter; regulation of apoptosis; response to antibiotic; negative regulation of cell differentiation; transforming growth factor beta receptor signaling pathway; response to cytokine stimulus; gene expression; spermatogenesis; positive regulation of axonogenesis; negative regulation of transforming growth factor beta receptor signaling pathway; cell cycle arrest; lymphocyte homeostasis