Mouse ADAM metallopeptidase domain 17 ELISA Kit | ADAM17 elisa kit

Mouse Disintegrin and metalloproteinase domain-containing protein 17, ADAM17 ELISA Kit

Cat. Num. AAA934765

• Gene Names: Adam17; Tace; CD156b
• Reactivity: Mouse
• Synonyms: ADAM metallopeptidase domain 17; Mouse Disintegrin and metalloproteinase domain-containing protein 17; ADAM17 ELISA Kit; Mouse Disintegrin and metalloproteinase domain-containing protein 17 (ADAM17) ELISA kit; ADAM18; CD156B; CSVP; MGC71942; TACE; ADAM metallopeptidase domain 18; TNF-alpha converting enzyme; a disintegrin and metalloprotease domain 17; snake venom-like protease; tumor necrosis factor; alpha; ; ADAM17 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse ADAM17. No significant cross-reactivity or interference between mouse ADAM17 and analogues was observed.
• Sequence Length: 827

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6 pg/ml-1000 pg/ml
• Sensitivity: < 3.9 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAM17 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAM17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAM17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM17 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM17 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 471270257
• NCBI GeneID: 11491
• NCBI Accession #: NP_001264195.1
• NCBI GenBank Nucleotide #: NM_001277266.1
• UniProt Accession #: Q9Z0F8; O88726; Q505A7; Q9R1U4; Q9Z0K3
• Molecular Weight: 93,056 Da
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 17 isoform 2
• NCBI Official Synonym Full Names: a disintegrin and metallopeptidase domain 17
• NCBI Official Symbol: Adam17
• NCBI Official Synonym Symbols: Tace; CD156b
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 17; TNF-alpha convertase; TNF-alpha converting enzyme; a disintegrin and metalloproteinase domain 17
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 17
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: Adam17
• UniProt Synonym Gene Names: Tace; ADAM 17
• UniProt Entry Name: ADA17_MOUSE

Uniprot Description

TACE: a type I membrane protein with disintegrin and metalloprotease activity. An ubiquitously expressed ectoenzyme of peptidase family M12B. Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Possesses a narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala-|-Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha) to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, and the amyloid precursor protein. Also involved in the activation of Notch pathway. Binds 1 zinc ion per subunit. Two splice variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Protease; Membrane protein, integral; EC 3.4.24.86

Cellular Component: cell surface; focal adhesion; membrane; integral to plasma membrane; cytoplasm; apical plasma membrane; extracellular region; plasma membrane; integral to membrane; intercellular junction; actin cytoskeleton; lipid raft

Molecular Function: integrin binding; peptidase activity; protein binding; interleukin-6 receptor binding; zinc ion binding; metallopeptidase activity; hydrolase activity; metalloendopeptidase activity; metal ion binding; Notch binding; SH3 domain binding; PDZ domain binding

Biological Process: cell motility involved in cell locomotion; T cell differentiation in the thymus; response to lipopolysaccharide; proteolysis; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; negative regulation of interleukin-8 production; germinal center formation; cell adhesion; epidermal growth factor receptor signaling pathway; response to drug; spleen development; Notch signaling pathway; response to high density lipoprotein stimulus; membrane protein ectodomain proteolysis; positive regulation of transforming growth factor beta receptor signaling pathway; Notch receptor processing; positive regulation of cell motility; positive regulation of cell growth; positive regulation of chemokine production; regulation of mast cell apoptosis; PMA-inducible membrane protein ectodomain proteolysis; B cell differentiation; response to hypoxia; cell adhesion mediated by integrin; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of apoptosis; positive regulation of cell migration