Rabbit anti-Human DUSP22 Polyclonal Antibody | anti-DUSP22 antibody

DUSP22 Polyclonal Antibody

Cat. Num. AAA3012028

• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: DUSP22; Polyclonal Antibody; DUSP22 Polyclonal Antibody; Dual specificity protein phosphatase 22; JNK-stimulatory phosphatase-1; JSP-1; Low molecular weight dual specificity phosphatase 2; LMW-DSP2; Mitogen-activated protein kinase phosphatase x; MAP kinase phosphatase x; MKP-x; JSP1; LMWDSP2; MKPX; anti-DUSP22 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DUSP22 polyclonal antibody detects endogenous levels of DUSP22 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 184

• Applicable Applications for anti-DUSP22 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant full length Human DUSP22.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of BT-474 cell line, using DUSP22 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF7 cell using DUSP22 antibody.)

Related Product Information for anti-DUSP22 antibody

Mitogen-activated protein (MAP) kinases are a large class of proteins involved in signal transduction pathways that are activated by a range of stimuli and mediate a number of physiological and pathological changes in the cell. Dual specificity phosphatases (DSPs) are a subclass of the protein tyrosine phosphatase (PTP) gene superfamily, which are selective for dephosphorylating critical phosphothreonine and phosphotyrosine residues within MAP kinases. DSP gene expression is induced by a host of growth factors and/or cellular stresses, thereby negatively regulating MAP kinase superfamily members including MAPK/ERK, SAPK/JNK and p38. DUSP22 dephosphorylates ERK2 MAP kinase and JNK. DUSP22 displays highest in thymus, but it is also detectable in monocytes and lymphocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 9910432
• NCBI Accession #: NP_064570.1
• NCBI GenBank Nucleotide #: NP_064570.1
• UniProt Accession #: Q9NRW4
• Molecular Weight: ~ 21kDa
• NCBI Official Full Name: dual specificity protein phosphatase 22 isoform b
• UniProt Protein Name: Dual specificity protein phosphatase 22
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP22
• UniProt Synonym Gene Names: JSP1; LMWDSP2; MKPX; JSP-1; LMW-DSP2; MAP kinase phosphatase x; MKP-x
• UniProt Entry Name: DUS22_HUMAN

Uniprot Description

DUSP22: Activates the Jnk signaling pathway. Dephosphorylates and deactivates p38 and stress-activated protein kinase/c-Jun N- terminal kinase (SAPK/JNK). Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; EC 3.1.3.16; Motility/polarity/chemotaxis; EC 3.1.3.48; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 6p25.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity

Biological Process: cell proliferation; apoptosis; transforming growth factor beta receptor signaling pathway; multicellular organismal development; positive regulation of JNK cascade; negative regulation of transcription from RNA polymerase II promoter; protein amino acid dephosphorylation; inactivation of MAPK activity; regulation of cell proliferation