Western Blot (WB)
(Western blot analysis of Phospho-SMYD2(Ser283) in lysates of NIH 3T3, using Phospho-SMYD2(Ser283) Antibody.)
Rabbit SMYD2 Polyclonal Antibody | anti-SMYD2 antibody
Phospho-SMYD2 (Ser283) Antibody
Predicted: Pig, Bovine, Horse, Rabbit, Dog, Chicken
Predicted: Pig, Bovine, Horse, Rabbit, Dog, Chicken
ELISA (Peptide): 1:20000-1:40000
Western Blot (WB)
(Western blot analysis of Phospho-SMYD2(Ser283) in lysates of NIH 3T3, using Phospho-SMYD2(Ser283) Antibody.)
Western Blot (WB)
(Western blot analysis of Phospho-SMYD2(Ser283) in lysates of NIH 3T3, using Phospho-SMYD2(Ser283) Antibody.)
Subunit Structure: Interacts with RNA polymerase II and HELZ. Interacts with SIN3A and HDAC1 (By similarity). Interacts (via MYND-type zinc finger) with EPB41L3. Interacts (via SET domain) with p53/TP53. Interacts with RB1 and HSP90AA1.
Similarity: Belongs to the class V-like SAM-binding methyltransferase superfamily.
NCBI and Uniprot Product Information
Predicted: 50 kDa
NCBI Description
SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]).[supplied by OMIM, Nov 2008]
Uniprot Description
SMYD2: Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Has also methyltransferase activity toward non-histone proteins such as p53/TP53 and RB1. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates 'Lys-860' of RB1/RB. Interacts with RNA polymerase II and HELZ. Interacts with SIN3A and HDAC1. Interacts (via MYND-type zinc finger) with EPB41L3. Interacts (via SET domain) with p53/TP53. Interacts with RB1 and HSP90AA1. Expression is repressed by CEBPA.
Protein type: Methyltransferase, protein lysine; EC 2.1.1.43; Methyltransferase
Chromosomal Location of Human Ortholog: 1q32.3
Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol
Molecular Function: protein binding; p53 binding; metal ion binding; protein-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K36 specific)
Biological Process: negative regulation of cell proliferation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; peptidyl-lysine di-methylation; peptidyl-lysine mono-methylation; histone H3-K36 methylation; negative regulation of transcription from RNA polymerase II promoter; regulation of DNA damage response, signal transduction by p53 class mediator
Research Articles on SMYD2
Similar Products
Product Notes
The SMYD2 smyd2 (Catalog #AAA9610453) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-SMYD2 (Ser283) Antibody reacts with Human, Mouse Predicted: Pig, Bovine, Horse, Rabbit, Dog, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's SMYD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 ELISA (Peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the SMYD2 smyd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMYD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
