Rabbit anti-Human SMYD1 Polyclonal Antibody | anti-SMYD1 antibody

SMYD1, CT (SMYD1, SET and MYND domain-containing protein 1) (AP)

Cat. Num. AAA6349155

• Gene Names: SMYD1; BOP; KMT3D; ZMYND18; ZMYND22
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SMYD1; Polyclonal Antibody; CT (SMYD1; SET and MYND domain-containing protein 1) (AP); SET and MYND domain-containing protein 1; anti-SMYD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-SMYD1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SMYD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 420-449 amino acids from the C-terminal region of human SMYD1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SMYD1 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SMYD1 antibody detected the SMYD1 protein (arrow).)

Flow Cytometry (FC/FACS)

(SMYD1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SMYD1 antibody

Acts as a transcriptional repressor. Essential for cardiomyocyte differentiation and cardiac morphogenesis (By similarity).

Product Categories/Family for anti-SMYD1 antibody

Antibodies; Cardiac Markers

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Yang, J., et al. J. Mol. Biol. 386(4):938-950(2009). Sims, R.J. III, et al. J. Biol. Chem. 277(29):26524-26529(2002). Gottlieb, P.D., et al. Nat. Genet. 31(1):25-32(2002). Srivastava, D., et al. Cold Spring Harb. Symp. Quant. Biol. 67, 121-125 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 38093643
• NCBI GeneID: 150572
• NCBI Accession #: NP_938015.1
• NCBI GenBank Nucleotide #: NM_198274
• UniProt Accession #: Q8NB12; A0AV30; A6NE13
• Molecular Weight: 56
• NCBI Official Full Name: histone-lysine N-methyltransferase SMYD1 isoform 1
• NCBI Official Synonym Full Names: SET and MYND domain containing 1
• NCBI Official Symbol: SMYD1
• NCBI Official Synonym Symbols: BOP; KMT3D; ZMYND18; ZMYND22
• NCBI Protein Information: histone-lysine N-methyltransferase SMYD1
• UniProt Protein Name: Histone-lysine N-methyltransferase SMYD1
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: SMYD1
• UniProt Entry Name: SMYD1_HUMAN

Uniprot Description

SMYD1: Acts as a transcriptional repressor. Essential for cardiomyocyte differentiation and cardiac morphogenesis.

Protein type: Methyltransferase, protein lysine; Transcription, coactivator/corepressor; EC 2.1.1.43

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; metal ion binding; histone-lysine N-methyltransferase activity; transcription corepressor activity

Biological Process: chromatin remodeling; transcription, DNA-dependent; heart development; negative regulation of transcription, DNA-dependent; positive regulation of myoblast differentiation