Mouse Receptor I For The Fc Region Of Immunoglobulin E (FceRI) ELISA Kit | FceRI elisa kit

Mouse Receptor I For The Fc Region Of Immunoglobulin E (FceRI) ELISA Kit

Cat. Num. AAA2703465

• Gene Names: MS4A2; APY; IGEL; IGER; ATOPY; FCERI; IGHER; MS4A1; FCER1B
• Reactivity: Mouse
• Synonyms: Receptor I For The Fc Region Of Immunoglobulin E (FceRI); Mouse Receptor I For The Fc Region Of Immunoglobulin E (FceRI) ELISA Kit; FCER1A; FCE1A; FcER1 High affinity immunoglobulin epsilon receptor subunit alpha; FceRI elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Receptor I For The Fc Region Of Immunoglobulin E (FceRI).; No significant cross-reactivity or interference between Receptor I For The Fc Region Of Immunoglobulin E (FceRI) and analogues was observed.
• Sequence Length: 152

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor I For The Fc Region Of Immunoglobulin E (FceRI) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor I For The Fc Region Of Immunoglobulin E (FceRI) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FceRI elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor I For The Fc Region Of Immunoglobulin E (FceRI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor I For The Fc Region Of Immunoglobulin E (FceRI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor I For The Fc Region Of Immunoglobulin E (FceRI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Receptor I For The Fc Region Of Immunoglobulin E (FceRI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 396464
• NCBI GeneID: 2206
• UniProt Accession #: Q14298; Q01362
• Molecular Weight: 16,454 Da
• NCBI Official Full Name: FceRI, partial
• NCBI Official Synonym Full Names: membrane spanning 4-domains A2
• NCBI Official Symbol: MS4A2
• NCBI Official Synonym Symbols: APY; IGEL; IGER; ATOPY; FCERI; IGHER; MS4A1; FCER1B
• NCBI Protein Information: high affinity immunoglobulin epsilon receptor subunit beta
• UniProt Protein Name: FceRI protein
• UniProt Gene Name: FceRI

NCBI Description

The allergic response involves the binding of allergen to receptor-bound IgE followed by cell activation and the release of mediators responsible for the manifestations of allergy. The IgE-receptor, a tetramer composed of an alpha, beta, and 2 disulfide-linked gamma chains, is found on the surface of mast cells and basophils. This gene encodes the beta subunit of the high affinity IgE receptor which is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This family member is localized to 11q12, among a cluster of membrane-spanning 4A gene family members. Alternative splicing results in multiple transcript variants encoding distinct proteins. Additional transcript variants have been described but require experimental validation. [provided by RefSeq, Mar 2012]