Mouse CD107b Monoclonal Antibody | anti-CD107b antibody

Mouse Anti Dog CD107b

Cat. Num. AAA225667

• Applications: Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot
• Purity: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Synonyms: CD107b; Monoclonal Antibody; Mouse Anti Dog CD107b; CD107b antibody (AC17); LAMP-2; anti-CD107b antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: AC17
• Specificity: CD107b
• Purity/Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Form/Format: Liquid; Phosphate Buffered Saline
• Concentration: Approx. Protien Concentrations: IgG concentration 1.0 mg/ml (varies by lot)

• Applicable Applications for anti-CD107b antibody: Flow Cytometry (FC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: FC: 1/50-1/200; WB: 1/100-1/500; ; Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.; ; Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.; (1) Membrane permeabilisation is required for this application.MyBioSource recommends the use of Leucoperm™ (Product Code MBS225936) for this purpose
• Immunogen: MDCK (Madin-Darby Canine Kidney) cells
• Target Species: Dog
• Species Cross Reactivity: Reacts with: Mink, Human; Does not react with: Mouse, Rat; N.B. Antibody reactivity and working conditions may vary between species. Crossreactivity is derived from testing within our laboratories, peer-reviewed publications orpersonal communications from the originators. Please refer to references indicated forfurther information.
• Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line.
• Preservative Stabilizers: 0.09% Sodium Azide (NaN3)
• Preparation and Storage: This product is shipped at ambient tempeture. It is recommended to aliquot and store at -20°C on receipt. When thawed , aliquot the sample as needed. Keep aliquots at 2°C to 8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C. ; Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-freeze freezers is not recommended.

Testing Data

(Peripheral dog granulocytes stained with Mouse anti Dog CD107b:RPE following permeabilisation with Leucoperm (BUF09).)

Testing Data

(Peripheral dog granulocytes stained with Mouse anti Dog CD107b following permeabilization with Leucoperm (BUF09). Goat anti Mouse IgG (H/L):FITC used as secondary antibody.)

Related Product Information for anti-CD107b antibody

Mouse anti Dog CD107b antibody, clone AC17 recognizes canine CD107b, otherwise known as lysosome-associated membrane protein 2 or LAMP-2. Immunofluorescence staining of MDCK cells with mouse anti dog CD107b, clone AC17 demonstrates staining patterns consistent with localization to lysozomes. This is supported by coincident staining of an exogenous lysozomal glycoprotein, avian LEP100 transfected into MDCK cells and detected using the anti LEP100 antibody clone CV24 (Nabi et al.1991).
Mouse anti Dog CD107b antibody, clone AC17 immunoprecipitates a protein of ~95 kDa in MDCK cells which, following Endo F digestion to remove N-linked oligosaccharides, yields a core protein product of 40 kDa, indicating the heavily glycosylated nature of CD107b. The molecular weight of canine CD107b is typical of many lysozome-associated membrane proteins. While most (97%) CD107b resides in the lysozomal environment in adherent MDCK cells in vitro, early studies (Nabi et al.1991) indicated that a small percentage of total cellular CD107b, as revealed by radioimmune assay with clone AC17, is found associated with the cell membrane.
Lysosomes are membrane-bound organelles found within the cytoplasm of most cells, they contain hydrolytic enzymes and act as the major compartment for heterophagic and autophagic digestion. Members of the lysosomal-associated membrane protein family (LAMPS) are believed to play an important role in protecting the lysosomal membrane from protease degradation and are involved in lectin-mediated cell adhesion. CD107b has been shown to share high N-terminal amino acid sequence homology with human, mouse and rat CD107b (Nabi et al.1993).
Transfection of a mink type II lung epithelial cell line with beta1-6-N-acetylglucosaminyl transferase V demonstrates the formation of large lysozomal vacuoles, termed multilamellar bodies (MLBs), having a very distinct phenotype with expression of CD107b, as indicated by immunofluorescent staining with clone AC17. These MLBs require lysozomal degradation via an autophagic pathway for their formation and may have implications for lysozomal storage diseases (Hariri et al.2000). Evidence shows that CD107b is involved in the lysosomal uptake of cytosolic proteins and the endocytic pathway. Human studies have revealed a correlation between the level of surface expression of CD107b on tumor cells and their metastatic potential (Saitoh et al. 1992).
Mouse anti Dog CD107b antibody, clone AC17 has been shown as suitable for use in electron microscopy (Nabi et al.1991).