Mouse anti-Rat N-CADHERIN Monoclonal Antibody | anti-Cdh2 antibody

MOUSE ANTI HUMAN N-CADHERIN

Cat. Num. AAA224676

• Gene Names: Cdh2; N-cadherin
• Reactivity: Rat
• Applications: Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot
• Synonyms: N-CADHERIN; Monoclonal Antibody; MOUSE ANTI HUMAN N-CADHERIN; anti-Cdh2 antibody

• Host: Mouse
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 13A9
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 906

• Applicable Applications for anti-Cdh2 antibody: Immunohistology Frozen, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 135-140kDa in human HT-1080 and HeLa cell lysates.; Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using steam heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 1/100; Maximum Dilution: 1/1000
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Immunogen: Recombinant MBP fusion protein containing the entire cytoplasmic domain of human N-cadherin.; Buffer Solution
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(HT1080 lysate probed with Mouse anti Human N-cadherin (MBS224676) followed by Rabbit F(ab')2 anti Mouse IgG:HRP (MBS235192))

Related Product Information for anti-Cdh2 antibody

Mouse anti Human N-cadherin antibody, clone 13A9 specifically recognizes neural cadherin, otherwise known as CD325, a calcium dependent cell-cell adhesion glycoprotein, and member of the cadherin superfamily, which links to the actin cytoskeleton via catenins, and plays a role in cell-matrix adhesion, cell growth and differentiation, and the establishment of left-right asymmetry. N-cadherin is expressed by neurons, endothelial cells, muscle cells, and stem cells, and is one of the primary cadherins recruited to the site of neuronal synapse formation. N-cadherin is directly involved in the differentiation of early hematopoietic progenitors, and is commonly expressed by cancer cells, playing a role in transendothelial migration and metastasis, through the up-regulation of the src kinase pathway, and subsequent failure of the intercellular connection between two adjacent endothelial cells. Mouse anti Human N-cadherin antibody, clone 13A9 studies  have demonstrated that expression levels of E-Cadherin and N-Cadherin have a role to play in the invasive properties of breast cancer. Decreased levels of E-cadherin and loss of E-cadherin-mediated adhesion, can result in the transition of a benign epithelial tumor to an invasive tumor, and increase invasiveness, whilst the expression of N-cadherin correlates with motility, invasiveness and tumor metastasis, irrespective of the presence of E-cadherin (Nieman et al. 1999). Mouse anti Human N-cadherin antibody, clone 13A9 has been shown to be specific for N-cadherin, and does not recognize E-cadherin, M-cadherin or P-cadherin (Knudsen et al. 1995). Immunohistological studies have shown that clone 13A9 can be used as a reliable marker for the differential identification of pleural mesotheliomas and lung adenocarcinomas, when used in conjunction with E-cadherin (Han et al. 1997).

NCBI and Uniprot Product Information

• NCBI GI #: 13786162
• NCBI GeneID: 83501
• NCBI Accession #: NP_112623.1
• NCBI GenBank Nucleotide #: NM_031333.1
• Molecular Weight: 99,686 Da
• NCBI Official Full Name: cadherin-2
• NCBI Official Synonym Full Names: cadherin 2
• NCBI Official Symbol: Cdh2
• NCBI Official Synonym Symbols: N-cadherin
• NCBI Protein Information: cadherin-2; cadherin 2 type 1 N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); neural cadherin
• UniProt Protein Name: Cadherin-2
• Protein Family: Cadherin
• UniProt Gene Name: Cdh2
• UniProt Synonym Gene Names: N-cadherin
• UniProt Entry Name: CADH2_RAT

NCBI Description

cell-cell adhesion molecule; may play a role in smooth muscle cell migration and adhesion in response to injury [RGD, Feb 2006]

Uniprot Description

CDH2: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. Interacts with CDCP1. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Interacts with PCDH8; this complex may also include TAOK2. The interaction with PCDH8 may lead to internalization through TAOK2/p38 MAPK pathway.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral

Cellular Component: protein complex; focal adhesion; basolateral plasma membrane; T-tubule; integral to membrane; fascia adherens; intercellular junction; catenin complex; adherens junction; cell-cell adherens junction; membrane; lamellipodium; apical plasma membrane; plasma membrane; synapse

Molecular Function: protein binding; enzyme binding; gamma-catenin binding; beta-catenin binding; protein complex binding; calcium ion binding; nitric-oxide synthase binding; protein phosphatase binding; protein kinase binding; alpha-catenin binding

Biological Process: cell migration; regulation of myelination; protein heterooligomerization; regulation of axonogenesis; heterophilic cell adhesion; cell-cell adhesion; regulation of protein localization; synaptogenesis; glial cell differentiation; positive regulation of MAPKKK cascade; calcium-dependent cell-cell adhesion; regulation of Rho protein signal transduction; striated muscle cell differentiation; blood vessel morphogenesis; cell adhesion; homophilic cell adhesion