Rabbit anti-Human SMG1 Polyclonal Antibody | anti-SMG1 antibody
SMG1, ID (Serine/Threonine-protein Kinase SMG1, SMG-1, hSMG-1, Lambda/iota Protein Kinase C-interacting Protein, Lambda-interacting Protein, 61E3.4, ATX, KIAA0421, LIP)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: ELISA: 1:1,000
Immunohistochemistry: 1:50-1:100
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein involved in nonsense-mediated mRNA decay (NMD) as part of the mRNA surveillance complex. The protein has kinase activity and is thought to function in NMD by phosphorylating the regulator of nonsense transcripts 1 protein. Alternatively spliced transcript variants have been described, but their full-length nature has yet to be determined. [provided by RefSeq, Mar 2013]
Uniprot Description
SMG1: Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ. Component of the SMG1C complex composed of SMG1, SMG8 and SMG9; the recruitment of SMG8 to SMG1 N-terminus induces a large conformational change in the SMG1 C-terminal head domain containing the catalytic domain. Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. Interacts with PRKCI. Interacts with TELO2 and TTI1. Interacts with RUVBL1 and RUVBL2. Interacts with UPF2. Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver. Inhibited by caffeine, LY294002 and wortmannin. Belongs to the PI3/PI4-kinase family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.11.1; Protein kinase, atypical; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; PIKK family; SMG1 subfamily
Chromosomal Location of Human Ortholog: 16p12.3
Cellular Component: cytoplasm; nucleus; cytosol
Molecular Function: protein dimerization activity; protein serine/threonine kinase activity; protein binding; metal ion binding; ATP binding; protein kinase activity
Biological Process: peptidyl-serine phosphorylation; mRNA export from nucleus; phosphoinositide phosphorylation; protein amino acid autophosphorylation; mRNA catabolic process, nonsense-mediated decay; response to stress; gene expression; double-strand break repair via homologous recombination
Research Articles on SMG1
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Product Notes
The SMG1 smg1 (Catalog #AAA629548) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SMG1, ID (Serine/Threonine-protein Kinase SMG1, SMG-1, hSMG-1, Lambda/iota Protein Kinase C-interacting Protein, Lambda-interacting Protein, 61E3.4, ATX, KIAA0421, LIP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMG1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Immunohistochemistry (IHC). Suitable for use in ELISA and Immunohistochemistry. Dilution: ELISA: 1:1,000 Immunohistochemistry: 1:50-1:100. Researchers should empirically determine the suitability of the SMG1 smg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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