Western Blot (WB)
(SMC3 antibody (pAb) tested by Western blot. Detection of SMC3 by Western blot analysis. Lane 1: Nuclear extract of HeLa cells (20 ug). Lane 2: Nuclear extract of Hep G2 cells (20 ug). Lane 3: Nuclear extract of HEK293 cells (20 ug). Lane 4: Nuclear extract of U2OS cells (20 ug). All were probed with SMC3 antibody (pAb) at a 1:500 dilution.)
Rabbit anti-Human SMC3 Polyclonal Antibody | anti-SMC3 antibody
SMC3 antibody (pAb)
WB: 1:500-1:1,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Western Blot (WB)
(SMC3 antibody (pAb) tested by Western blot. Detection of SMC3 by Western blot analysis. Lane 1: Nuclear extract of HeLa cells (20 ug). Lane 2: Nuclear extract of Hep G2 cells (20 ug). Lane 3: Nuclear extract of HEK293 cells (20 ug). Lane 4: Nuclear extract of U2OS cells (20 ug). All were probed with SMC3 antibody (pAb) at a 1:500 dilution.)
Western Blot (WB)
(SMC3 antibody (pAb) tested by Western blot. Detection of SMC3 by Western blot analysis. Lane 1: Nuclear extract of HeLa cells (20 ug). Lane 2: Nuclear extract of Hep G2 cells (20 ug). Lane 3: Nuclear extract of HEK293 cells (20 ug). Lane 4: Nuclear extract of U2OS cells (20 ug). All were probed with SMC3 antibody (pAb) at a 1:500 dilution.)
Background: The Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. SMC3 is a component of the cohesin complex that plays an essential role during chromatid segregation. SMC3 is also involved in DNA recombination and repair, mitotic chromosome organization and microtubule-mediated intracellular transport. SMC3 is acetylated during replication, which is essential for cohesion, and is phosphorylated by ATM subsequent to DNA double strand breakage. SMC3 is frequently elevated in human colon carcinoma and overexpression of the protein transforms fibroblasts.
NCBI and Uniprot Product Information
Uniprot Description
SMC3: Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement. Interacts with MXI1, MXD3 and MXD4. Interacts with SYCP2. Found in a complex with SMC1A, CDCA5 and RAD21, PDS5A/APRIN and PDS5B/SCC-112. Forms a heterodimer with SMC1A or SMC1B in cohesin complexes. Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. Also found in meiosis-specific cohesin complexes. Interacts with NUMA1, and forms a ternary complex with KIF3B and KIFAP3, suggesting a function in tethering the chromosomes to the spindle pole and in chromosome movement. Interacts with PDS5A and WAPAL; regulated by SMC3 acetylation. Belongs to the SMC family. SMC3 subfamily.
Protein type: Cell cycle regulation; DNA repair, damage
Chromosomal Location of Human Ortholog: 10q25
Cellular Component: cohesin complex; spindle pole; nuclear matrix; cohesin core heterodimer; chromosome; cytosol; nucleoplasm; lateral element; cytoplasm; basement membrane; chromatin; nucleus; chromosome, pericentric region
Molecular Function: dynein binding; protein binding; protein heterodimerization activity; microtubule motor activity; chromatin binding; ATP binding
Biological Process: mitosis; meiosis; mitotic spindle organization and biogenesis; cell division; mitotic sister chromatid cohesion; stem cell maintenance; sister chromatid cohesion; negative regulation of DNA endoreduplication; mitotic cell cycle; signal transduction; DNA repair; regulation of DNA replication
Disease: Cornelia De Lange Syndrome 3
Similar Products
Product Notes
The SMC3 smc3 (Catalog #AAA388774) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SMC3 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMC3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:500-1:1,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the SMC3 smc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
