Rabbit anti-Human SMARCAL1 Polyclonal Antibody | anti-SMARCAL1 antibody

Rabbit Polyclonal to Human SMARCAL1

Cat. Num. AAA240512

• Gene Names: SMARCAL1; HARP; HHARP
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: SMARCAL1; Polyclonal Antibody; Rabbit Polyclonal to Human SMARCAL1; Anti-SMARCAL1 Antibody (aa80-96) IHC-plus; ATP-driven annealing helicase; HARP; HepA-related protein; HHARP; SMARCA-like protein 1; SIOD; Human SMARCAL1; anti-SMARCAL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: aa 80-96 of human SmarcAL1 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 954

• Applicable Applications for anti-SMARCAL1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:16000 - 1:64000), IHC-P (5 ug/ml), WB (1:500 - 1:3000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human SMARCAL1.
• Immunogen Type: Synthetic peptide
• Immunogen: SMARCAL1 antibody was raised against synthetic peptide from human SMARCAL1.
• Antigen Modification: aa80-96
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-SMARCAL1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Anti-SmarcAL1 Antibody - Western Blot. Western blot of Affinity Purified anti-SmarcAL1 antibody shows detection of a band ~106 kD band (arrowhead) corresponding to SmarcAL1 in human derived cultured cell lysates (HeLa nuclear extract lane 1, HeLa lane 2, A431 lane 3, Jurkat lane 4, and 293 lane 5). Approximately 5 ug of each lysates was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-SmarcAL1 antibody. Signal was detected using standard techniques. SmarcAL1 is the band seen between the 97 and 116kD markers.)

NCBI and Uniprot Product Information

• NCBI GI #: 21071060
• NCBI GeneID: 50485
• NCBI Accession #: NP_054859.2
• NCBI GenBank Nucleotide #: NM_014140.3
• UniProt Accession #: Q9NZC9; Q53R00; Q96AY1; Q9NXQ5; Q9UFH3; Q9UI93; A6NEH0
• Molecular Weight: 105,938 Da
• NCBI Official Full Name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
• NCBI Official Synonym Full Names: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1
• NCBI Official Symbol: SMARCAL1
• NCBI Official Synonym Symbols: HARP; HHARP
• NCBI Protein Information: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1; HepA-related protein; SMARCA-like protein 1; ATP-driven annealing helicase; sucrose nonfermenting protein 2-like 1
• UniProt Protein Name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
• UniProt Gene Name: SMARCAL1
• UniProt Synonym Gene Names: HARP; hHARP
• UniProt Entry Name: SMAL1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

SMARCAL1: ATP-dependent annealing helicase that catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. Defects in SMARCAL1 are a cause of Schimke immuno-osseous dysplasia (SIOD). SIOD causes spondyloepiphyseal dysplasia, renal dysfunction and T-cell immunodeficiency. Approximately half of all patients also exhibit hyperthyroidism, while around half also exhibit episodal cerebral ischema. Belongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily.

Protein type: EC 3.6.4.-; Helicase; EC 3.6.1.-

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: nucleoplasm; DNA replication factor A complex; nucleus

Molecular Function: DNA-dependent ATPase activity; protein binding; DNA binding; helicase activity; ATP binding

Biological Process: regulation of transcription from RNA polymerase II promoter; DNA strand renaturation; chromatin modification; DNA repair; response to DNA damage stimulus; DNA metabolic process; replication fork processing

Disease: Immunoosseous Dysplasia, Schimke Type