Western Blot (WB)
(Western blot analysis of RhoA expression in HepG2 (A), MCF7 (B), HL60 (C) whole cell lysates.)
Rabbit RhoA Polyclonal Antibody | anti-RHOA antibody
Anti-RhoA Antibody
Western Blot (WB)
(Western blot analysis of RhoA expression in HepG2 (A), MCF7 (B), HL60 (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of RhoA expression in HepG2 (A), MCF7 (B), HL60 (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of RhoA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of RhoA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
Uniprot Description
RHOA: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.
Protein type: G protein, monomeric; G protein, monomeric, Rho; G protein; Motility/polarity/chemotaxis; Oncoprotein
Chromosomal Location of Human Ortholog: 3p21.3
Cellular Component: apical junction complex; cell cortex; cell junction; cleavage furrow; cytoskeleton; cytosol; endoplasmic reticulum membrane; endosome; focal adhesion; lamellipodium; midbody; plasma membrane; vesicle
Molecular Function: GTP binding; GTPase activity; myosin binding; protein binding
Biological Process: actin cytoskeleton organization and biogenesis; apical junction assembly; axon guidance; blood coagulation; endothelial cell migration; ephrin receptor signaling pathway; negative chemotaxis; negative regulation of axonogenesis; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; platelet activation; positive regulation of axonogenesis; positive regulation of cytokinesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of lipase activity; positive regulation of neuron differentiation; positive regulation of NF-kappaB import into nucleus; positive regulation of stress fiber formation; regulation of actin cytoskeleton organization and biogenesis; regulation of axonogenesis; regulation of cell migration; regulation of osteoblast proliferation; regulation of small GTPase mediated signal transduction; Rho protein signal transduction; small GTPase mediated signal transduction; substantia nigra development; transforming growth factor beta receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; viral reproduction
Research Articles on RHOA
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Product Notes
The RHOA rhoa (Catalog #AAA8241654) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RhoA Antibody reacts with Human, Bovine, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's RhoA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the RHOA rhoa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RhoA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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