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Western Blot (WB) (Western blot analysis of DNAL4 using HepG2 whole cell lysates)

Rabbit anti-Human, Mouse DNAL4 Polyclonal Antibody | anti-DNAL4 antibody

DNAL4 Antibody

Gene Names
DNAL4; MRMV3; PIG27
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
DNAL4; Polyclonal Antibody; DNAL4 Antibody; D15Ertd424e; Dnal4; DNAL4_HUMAN; Dnalc4; Dynein axonemal light chain 4; Dynein light chain 4; axonemal; Dynein light chain; outer arm 4; Dynein; light 4; light polypeptide 4; MGC105573; PIG27; Proliferation inducing gene 27; Proliferation inducing protein 27; anti-DNAL4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
DNAL4 antibody detects endogenous levels of total DNAL4
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
105
Applicable Applications for anti-DNAL4 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
Immunogen
A synthesized peptide derived from human DNAL4
Subcellular Location
Cytoskeleton
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of DNAL4 using HepG2 whole cell lysates)

Western Blot (WB) (Western blot analysis of DNAL4 using HepG2 whole cell lysates)

Immunohistochemistry (IHC)

(MBS9609787 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Immunohistochemistry (IHC) (MBS9609787 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-DNAL4 antibody
Function: Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity (By similarity).
Subunit Structure: Consists of at least two heavy chains and a number of intermediate and light chains.
Similarity: Belongs to the dynein light chain family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 12 kDa
Predicted: 13 kDa
NCBI Official Full Name
dynein light chain 4, axonemal
NCBI Official Synonym Full Names
dynein axonemal light chain 4
NCBI Official Symbol
DNAL4
NCBI Official Synonym Symbols
MRMV3; PIG27
NCBI Protein Information
dynein light chain 4, axonemal
UniProt Protein Name
Dynein light chain 4, axonemal
Protein Family
UniProt Gene Name
DNAL4

NCBI Description

This gene encodes an axonemal dynein light chain which functions as a component of the outer dynein arms complex. This complex acts as the molecular motor that provides the force to move cilia in an ATP-dependent manner. The encoded protein is expressed in tissues with motile cilia or flagella and may be involved in the movement of sperm flagella. [provided by RefSeq, Dec 2014]

Uniprot Description

Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity ().

Research Articles on DNAL4

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Product Notes

The DNAL4 dnal4 (Catalog #AAA9609787) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNAL4 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DNAL4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the DNAL4 dnal4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAL4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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