Testing Data
(Western Blot analysis of extracts from Hela cells untreated or treated with PMA using Smad2 (Phospho-Ser467) antibody)
Rabbit SMAD2 Polyclonal Antibody | anti-SMAD2 antibody
SMAD2 antibody
Applications
Western Blot
Purity
Purified by immunoaffinity chromatography.
Synonyms
SMAD2; Polyclonal Antibody; SMAD2 antibody; Rabbit polyclonal SMAD2 antibody; Polyclonal SMAD2 antibody; Anti-SMAD2 antibody; MADH2 antibody; SMAD-2 antibody; Mothers against DPP homolog 2 antibody; MADR2 antibody; SMAD 2; SMAD 2 antibody; SMAD family member 2 antibody; Phospho Ser467 antibody; SMAD-2; anti-SMAD2 antibody
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl,0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
467
Applicable Applications for anti-SMAD2 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
SMAD2 antibody was raised in Rabbit using the peptide sequence around phosphorylation site of serine 467 (C-S-S-M-S(p)) derived from Human Smad2 as the immunogen.
Cross-Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at 4 degree C for short term storage.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Testing Data
(Western Blot analysis of extracts from Hela cells untreated or treated with PMA using Smad2 (Phospho-Ser467) antibody)
Testing Data
(Western Blot analysis of extracts from Hela cells untreated or treated with PMA using Smad2 (Phospho-Ser467) antibody)
Related Product Information for anti-SMAD2 antibody
SMAD2 belongs to the SMAD, a family of proteins similar to the gene products of theDrosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signaltransducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signalof the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cellproliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through itsinteraction with the SMAD anchor for receptor activation (SARA) protein.
Product Categories/Family for anti-SMAD2 antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
48,956 Da
NCBI Official Full Name
Smad2
UniProt Protein Name
Mothers against decapentaplegic homolog 2
Protein Family
UniProt Gene Name
SMAD2
UniProt Synonym Gene Names
MADH2; MADR2; MAD homolog 2; Mothers against DPP homolog 2; hMAD-2; SMAD 2; Smad2; hSMAD2
Uniprot Description
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Similar Products
Product Notes
The SMAD2 smad2 (Catalog #AAA5309619) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SMAD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the SMAD2 smad2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMAD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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