Typical Testing Data/Standard Curve (for reference only)
Human Cohesin subunit SA 1 (STAG1) ELISA Kit | STAG1 elisa kit
Human Cohesin subunit SA 1 (STAG1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: STAG1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-STAG1 antibody and an STAG1-HRP conjugate. The assay sample and buffer are incubated together with STAG1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the STAG1 concentration since STAG1 from samples and STAG1-HRP conjugate compete for the anti-STAG1 antibody binding site. Since the number of sites is limited, as more sites are occupied by STAG1 from the sample, fewer sites are left to bind STAG1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STAG1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase. [provided by RefSeq, Jul 2008]
Uniprot Description
STAG1: Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Belongs to the SCC3 family.
Protein type: Apoptosis
Chromosomal Location of Human Ortholog: 3q22.3
Cellular Component: nucleoplasm; chromosome; cytosol; nucleus; chromatin; chromosome, pericentric region
Molecular Function: protein binding; chromatin binding
Biological Process: mitosis; cell division; mitotic cell cycle; chromosome segregation
Research Articles on STAG1
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Product Notes
The Human STAG1 stag1 (Catalog #AAA7247600) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7247600 ELISA Kit recognizes Human STAG1. It is sometimes possible for the material contained within the vial of "Cohesin subunit SA 1 (STAG1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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