Rabbit SLC9A3R1 Polyclonal Antibody | anti-SLC9A3R1 antibody

SLC9A3R1 Antibody

Cat. Num. AAA9608672

• Gene Names: SLC9A3R1; EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: SLC9A3R1; Polyclonal Antibody; SLC9A3R1 Antibody; EBP 50; EBP50; Ezrin radixin moesin binding phosphoprotein 50; Ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor NHE RF; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co factor; NHERF 1; NHERF; NHERF-1; NHERF1; NHRF1_HUMAN; NPHLOP2; Regulatory cofactor of Na(+)/H(+) exchanger; Slc9a3r1; Sodium hydrogen exchanger regulatory factor 1; Sodium-hydrogen exchanger regulatory factor 1; Sodium/hydrogen exchanger regulatory factor 1; Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1; Solute carrier family 9 (sodium/hydrogen exchanger); isoform 3 regulatory factor 1; Solute carrier family 9 isoform 3 regulatory factor 1; Solute carrier family 9 isoform A3 regulatory factor 1; Solute carrier family 9 member 3 regulator 1; anti-SLC9A3R1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SLC9A3R1 antibody detects endogenous levels of total SLC9A3R1
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 358

• Applicable Applications for anti-SLC9A3R1 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:1000-3000; IHC: 1:200
• Immunogen: A synthesized peptide derived from human SLC9A3R1
• Subcellular Location: Cytoplasm. Apical Cell Membrane. Endomembrane System. Cell Projection > Filopodium. Cell Projection > Ruffle. Cell Projection > Microvillus. Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T-cells.
• Tissue Specificity: Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.
• Predicted Cross Reactivity: Bovine, Horse, Sheep, Rabbit, Dog
• Similarity: Bovine (100%), Horse (100%), Sheep (100%), Rabbit (92%), Dog (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9608672 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis SLC9A3R1 using COLO205 whole cell lysates)

Related Product Information for anti-SLC9A3R1 antibody

Function: Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa.
Subunit Structure: Homodimer, and heterodimer with SLC9A3R2. Binds the N-termini of EZR, RDX and MSN. Binds the C-termini of PDGFRA, PDGFRB, ADRB2, NOS2 and CFTR. Binds ARHGAP17, EPI64, RACK1, OPRK1, GNAQ, CTNNB1 and PLCB3. Binds PDZK1 (By similarity). Interacts with CLCN3. Binds the C-terminus of PAG1. In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation. Forms a complex with CFTR and SLC4A7. Forms a complex with SLC4A7 and ATP6V1B1. Interacts with TRPC4 (via the PDZ-binding domain). Directly interacts with HTR4 (By similarity). Interacts (via the PDZ 1 domain) with PODXL (via the C-terminal PDZ-binding motif DTHL); interaction is not detected in glomerular epithelium cells. Interacts (via the PDZ 1 domain) with PODXL (via the C-terminal PDZ-binding motif DTHL); the interaction take place early in the secretory pathway and is necessary for its apical membrane sorting (By similarity). Interacts with SLC26A3 (By similarity). Interacts with MCC. Interacts with SLC34A1. Interacts (via the PDZ domains) with SLC26A6 isoform 4 and isoform 5.
Post-translational Modifications: Phosphorylated on serine residues.

References

Ying-yuLu, JianLiang, Shu-XianChen, Bing-xinWang, HanYuan, Can-taoLi, Ya-yunWu, Yan-fangWu, Xu-guangShi, JieGao, Shao-zhenHou; Journal: J FUNCT FOODS. Phloridzin alleviate colitis in mice by protecting the intestinal brush border and improving the expression of sodium glycogen transporter 1.

NCBI and Uniprot Product Information

• NCBI GI #: 4759140
• NCBI GeneID: 9368
• NCBI Accession #: NP_004243.1
• NCBI GenBank Nucleotide #: NM_004252.4
• UniProt Accession #: O14745; O43552; Q86WQ5; B3KY21
• Molecular Weight: Observed: 39 kDa; Predicted: 39 kDa
• NCBI Official Full Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• NCBI Official Synonym Full Names: SLC9A3 regulator 1
• NCBI Official Symbol: SLC9A3R1
• NCBI Official Synonym Symbols: EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2
• NCBI Protein Information: Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• UniProt Protein Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• Protein Family: Na(+)/H(+) exchange regulatory cofactor
• UniProt Gene Name: SLC9A3R1
• UniProt Synonym Gene Names: NHERF; NHERF1; NHERF-1; EBP50

NCBI Description

This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer.[provided by RefSeq, Sep 2009]

Uniprot Description

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (). Involved in the regulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa.