Rabbit anti-Human SLC7A8 Polyclonal Antibody | anti-SLC7A8 antibody

SLC7A8 Antibody (C-Term)

Cat. Num. AAA9231062

• Gene Names: SLC7A8; LAT2; LPI-PC1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Synonyms: SLC7A8; Polyclonal Antibody; SLC7A8 Antibody (C-Term); Large neutral amino acids transporter small subunit 2; L-type amino acid transporter 2; hLAT2; Solute carrier family 7 member 8; LAT2; anti-SLC7A8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This SLC7A8 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 476-506 amino acids from human SLC7A8.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 430

• Applicable Applications for anti-SLC7A8 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-1:2000
• Antigen Type: Synthesized Peptide
• Antigen Source: Human
• Tissue Location: Strongest expression is observed in kidney and moderate expression in placenta and brain, followed by liver, prostate, testis, ovary, lymph node, thymus, spleen, skeletal muscle and heart. Also expressed in fetal liver as well as in the retinal pigment epithelial cell line ARPE-19 and the intestinal epithelial cell line Caco-2.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-SLC7A8 Antibody (C-Term) at 1:1000-1:2000 dilutionLane 1: Human brain lysateLane 2: Human kidney lysateLane 3: Human liver lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-SLC7A8 antibody

Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.

Product Categories/Family for anti-SLC7A8 antibody

Primary Antibodies; Metabolism; Neuroscience; Signal Transduction

References

Pineda M.,et al.J. Biol. Chem. 274:19738-19744 (1999). Rossier G.,et al.J. Biol. Chem. 274:34948-34954 (1999). Borsani G.,et al.Nat. Genet. 21:297-301 (1999). Park S.Y.,et al.Arch. Pharm. Res. 28:421-432 (2005). Li W.B.,et al.Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 388423198
• NCBI GeneID: 23428
• NCBI Accession #: NP_001253965.1
• NCBI GenBank Nucleotide #: NM_001267036.1
• UniProt Accession #: Q9UHI5; Q86U05; Q9UKQ6; Q9UKQ7; Q9UKQ8; Q9Y445; B2R8Q4; B4DKT4; B4DTV6; D3DS46; F2Z2J4
• Molecular Weight: 58382 Da
• NCBI Official Full Name: large neutral amino acids transporter small subunit 2 isoform c
• NCBI Official Synonym Full Names: solute carrier family 7 member 8
• NCBI Official Symbol: SLC7A8
• NCBI Official Synonym Symbols: LAT2; LPI-PC1
• NCBI Protein Information: large neutral amino acids transporter small subunit 2
• UniProt Protein Name: Large neutral amino acids transporter small subunit 2
• Protein Family: Large neutral amino acids transporter
• UniProt Gene Name: SLC7A8
• UniProt Synonym Gene Names: LAT2; hLAT2

Uniprot Description

SLC7A8: Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney. Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: amino acid transmembrane transporter activity; antiporter activity; L-amino acid transmembrane transporter activity; neutral amino acid transmembrane transporter activity; organic cation transmembrane transporter activity; peptide antigen binding; protein binding; toxin transporter activity

Biological Process: amino acid metabolic process; amino acid transport; leukocyte migration; neutral amino acid transport; transport