Western Blot (WB)
(MBS421351(2ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. )
Goat SLC6A4/5HTT/SERT Polyclonal Antibody | anti-SLC6A4 antibody
Goat anti-SLC6A4/5HTT/SERT Antibody
Western Blot: Approx 70-75kDa band observed in Human Colon lysates (calculated MW of 70.3kDa according to NP_001036.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.
Immunofluorescence: Strong expression of the protein seen in vesicles of HepG2 cells. Recommended concentration: 10ug/ml
Western Blot (WB)
(MBS421351(2ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. )
Western Blot (WB)
(MBS421351(2ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. )
Immunofluorescence (IF)
(MBS421351 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Immunofluorescence (IF)
(MBS421351 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. The encoded protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is a target of psychomotor stimulants, such as amphetamines and cocaine, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, and depression-susceptibility in people experiencing emotional trauma. [provided by RefSeq, Jul 2008]
Uniprot Description
SERT: Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.
Protein type: Membrane protein, multi-pass; Transporter; Membrane protein, integral
Chromosomal Location of Human Ortholog: 17q11.2
Cellular Component: cytosol; endomembrane system; endosome membrane; integral to plasma membrane; lipid raft; neuron projection; plasma membrane
Molecular Function: actin filament binding; cocaine binding; metal ion binding; monoamine transmembrane transporter activity; myosin binding; nitric-oxide synthase binding; protein binding; protein homodimerization activity; Rab GTPase binding; serotonin transmembrane transporter activity; serotonin:sodium symporter activity; syntaxin-1 binding
Biological Process: brain morphogenesis; circadian rhythm; memory; monoamine transport; negative regulation of granule cell precursor proliferation; negative regulation of neuron differentiation; negative regulation of organ growth; negative regulation of synaptic transmission, dopaminergic; positive regulation of cell cycle; protein homooligomerization; protein oligomerization; response to drug; response to estradiol stimulus; response to hypoxia; response to nutrient; response to toxin; serotonin transport; serotonin uptake; social behavior; sperm ejaculation; synaptic transmission; thalamus development; transmembrane transport; vasoconstriction
Disease: Anxiety; Obsessive-compulsive Disorder
Research Articles on SLC6A4
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Product Notes
The SLC6A4 slc6a4 (Catalog #AAA421351) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SLC6A4/5HTT/SERT Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SLC6A4/5HTT/SERT can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 70-75kDa band observed in Human Colon lysates (calculated MW of 70.3kDa according to NP_001036.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in vesicles of HepG2 cells. Recommended concentration: 10ug/ml. Researchers should empirically determine the suitability of the SLC6A4 slc6a4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC6A4/5HTT/SERT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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