Immunohistochemistry (IHC)
(Anti-SLC6A12 / BGT-1 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Goat SLC6A12 / BGT-1 Polyclonal Antibody | anti-SLC6A12 antibody
Goat Polyclonal to Human SLC6A12 / BGT-1
Gene Names
SLC6A12; BGT1; GAT2; BGT-1
Reactivity
Chimpanzee, Gorilla, Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Immunoaffinity Purified
Synonyms
SLC6A12 / BGT-1; Polyclonal Antibody; Goat Polyclonal to Human SLC6A12 / BGT-1; Anti-SLC6A12 / BGT-1 Antibody (C-Terminus) IHC-plus; SLC6A12; BGT; BGT-1; BGT1; Gat2 mouse; Betaine transporter; GAT2; Human SLC6A12; anti-SLC6A12 antibody
Host
Goat
Reactivity
Chimpanzee, Gorilla, Human
Clonality
Polyclonal
Specificity
Human SLC6A12 / BGT-1. Reported variants represent identical protein: NP_001193860.1, NP_003035.3, NP_001116319.1, NP_001116320.1.
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
614
Applicable Applications for anti-SLC6A12 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA (1:128000), IHC-P (10 ug/ml), WB (1 - 3 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 70kD band observed in Human Brain lysates from several regions (calculated MW of 69.4kD according to NP_003035.3). Recommended concentration: 1-3 ug/ml. An additional ban...
Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 70kD band observed in Human Brain lysates from several regions (calculated MW of 69.4kD according to NP_003035.3). Recommended concentration: 1-3 ug/ml. An additional ban...
Target Species
Human
Immunogen Description
Synthetic peptide CLDGSAGRNFGPSPT from the C-terminus of human SLC6A12 / BGT-1 (NP_003035.3). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Orangutan (87%).
Immunogen Type
Synthetic peptide
Immunogen
SLC6A12 / BGT-1 antibody was raised against synthetic peptide CLDGSAGRNFGPSPT from the C-terminus of human SLC6A12 / BGT-1 (NP_003035.3). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Orangutan (87%).
Antigen Modification
C-Terminus
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Immunohistochemistry (IHC)
(Anti-SLC6A12 / BGT-1 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunohistochemistry (IHC)
(Anti-SLC6A12 / BGT-1 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Western Blot (WB)
(SLC6A12 antibody (1 ug/ml) staining of Human Olfactory bulb lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(SLC6A12 antibody (1 ug/ml) staining of Human Olfactory bulb lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Product Categories/Family for anti-SLC6A12 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69,368 Da
NCBI Official Full Name
sodium- and chloride-dependent betaine transporter
NCBI Official Synonym Full Names
solute carrier family 6 (neurotransmitter transporter), member 12
NCBI Official Symbol
SLC6A12
NCBI Official Synonym Symbols
BGT1; GAT2; BGT-1
NCBI Protein Information
sodium- and chloride-dependent betaine transporter; betaine/GABA transporter-1; gamma-aminobutyric acid transporter; Na(+)/Cl(-) betaine/GABA transporter; solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12
UniProt Protein Name
Sodium- and chloride-dependent betaine transporter
UniProt Gene Name
SLC6A12
UniProt Entry Name
S6A12_HUMAN
Uniprot Description
SLC6A12: Transports betaine and GABA. May have a role in regulation of GABAergic transmission in the brain through the reuptake of GABA into presynaptic terminals, as well as in osmotic regulation. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A12 subfamily.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, SLC family
Chromosomal Location of Human Ortholog: 12p13
Cellular Component: integral to plasma membrane; plasma membrane; integral to membrane
Molecular Function: gamma-aminobutyric acid:sodium symporter activity; neurotransmitter:sodium symporter activity
Biological Process: response to organic substance; synaptic transmission; cellular water homeostasis; transport; amino acid transport; neurotransmitter secretion; ion transport; transmembrane transport; gamma-aminobutyric acid transport
Research Articles on SLC6A12
Similar Products
Product Notes
The SLC6A12 slc6a12 (Catalog #AAA248410) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Human SLC6A12 / BGT-1 reacts with Chimpanzee, Gorilla, Human and may cross-react with other species as described in the data sheet. AAA Biotech's SLC6A12 / BGT-1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA (1:128000), IHC-P (10 ug/ml), WB (1 - 3 ug/ml) Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 70kD band observed in Human Brain lysates from several regions (calculated MW of 69.4kD according to NP_003035.3). Recommended concentration: 1-3 ug/ml. An additional ban.. Researchers should empirically determine the suitability of the SLC6A12 slc6a12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC6A12 / BGT-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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