Immunofluorescence (IF)
(Immunofluorescence staining of SLC26A11 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human SLC26A11 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Rabbit anti-Human SLC26A11 Polyclonal Antibody | anti-SLC26A11 antibody
Anti-SLC26A11 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
SLC26A11; Polyclonal Antibody; Anti-SLC26A11 Antibody; Rabbit Polyclonal; SLC26A11 Antibody; Rabbit PAb; Antigen Affinity Purified; solute carrier family 26 member 11; anti-SLC26A11 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human SLC26A11
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-SLC26A11 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ICC/IF: 1:100-1:500
Immunogen
E Coli-derived Human SLC26A11 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human SLC26A11 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of SLC26A11 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human SLC26A11 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of SLC26A11 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human SLC26A11 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Related Product Information for anti-SLC26A11 antibody
Solute-linked carrier 26 (SLC26) isoforms are members of a large, conserved family of anion exchangers, many of which display highly restricted and distinct tissue distribution. Cloning experiments have identified 10 SLC26 genes or isoforms (SLC26A1-11). Except for SLC26A5 (prestin), all function as anion exchangers with versatility with respect to transported anions. Modes of transport mediated by SLC26 members include the exchange of chloride for bicarbonate, hydroxyl, sulfate, formate, iodide, or oxalate with variable specificity. Other anion exchange modes not involving chloride also have been reported for some of the members of this family. Several members of SLC26 isoforms are expressed in the kidney. These include SLC26A1 (SAT1), SLC26A4 (pendrin), SLC26A6, SLC26A7, and SLC26A11. SLC26A11 (human)/Slc26a11 (mouse), also known as kidney brain anion transporter (KBAT), is a member of the SLC26 anion transporter family and shows abundant mRNA expression in the brain.
References
Soleimani M, et al. (2006) Slc26 chloride/base exchangers in the kidney in health and disease. Semin Nephrol 26 (5): 375-385.
Soleimani M (2013) Slc26 cl-/hco3-exchangers in the kidney: Roles in health and disease. Kidney Int 84 (4): 657-666.
Rahmati N, et al. (2013) Slc26a11 is prominently expressed in the brain and functions as a chloride channel: Expression in purkinje cells and stimulation of v h+-atpase. Pflugers Arch 465 (11): 1583-1597.
Soleimani M (2013) Slc26 cl-/hco3-exchangers in the kidney: Roles in health and disease. Kidney Int 84 (4): 657-666.
Rahmati N, et al. (2013) Slc26a11 is prominently expressed in the brain and functions as a chloride channel: Expression in purkinje cells and stimulation of v h+-atpase. Pflugers Arch 465 (11): 1583-1597.
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Product Notes
The SLC26A11 (Catalog #AAA8109033) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SLC26A11 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SLC26A11 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:100-1:500. Researchers should empirically determine the suitability of the SLC26A11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC26A11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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