Rabbit SLC22A2 Polyclonal Antibody | anti-SLC22A2 antibody

SLC22A2 Polyclonal Antibody

Cat. Num. AAA3006729

• Gene Names: SLC22A2; OCT2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SLC22A2; Polyclonal Antibody; SLC22A2 Polyclonal Antibody; Solute carrier family 22 member 2; Organic cation transporter 2; hOCT2; OCT2; anti-SLC22A2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SLC22A2 polyclonal antibody detects endogenous levels of SLC22A2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 555

• Applicable Applications for anti-SLC22A2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human SLC22A2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SLC22A2 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:sp2/0 cell lysateLane3:H9C2 cell lysate)

Related Product Information for anti-SLC22A2 antibody

Organic cation transporters (OCT) are expressed in the plasma membrane of epithelial cells from a wide range of tissues, where they function in the elimination of endogenous amines, cationic drugs and other xenobiotics. The structure of OCTs consists of a 12-transmembrane-domain structure and a large extracellular hydrophilic loop. In humans, OCT1 is primarily expressed in the liver, while OCT2 is expressed in the kidney. OCT3 is expressed in the placenta, skeletal muscle, prostate, aorta and liver. OCT2, also known as SLC22A2, is a multi-specific transporter protein localizing to the basolateral and luminal membranes of the kidney distal tubule and proximal tubules. OCT2 is responsible for mediating the pH-sensitive tubular uptake of organic compounds from circulation. An additional splice variant exists for OCT2, namely OCT2-A.

NCBI and Uniprot Product Information

• NCBI GI #: 302148518
• NCBI GeneID: 6582
• NCBI Accession #: NP_003049.2
• NCBI GenBank Nucleotide #: NP_003049.2
• UniProt Accession #: O15244
• Molecular Weight: ~ 100kDa
• NCBI Official Full Name: solute carrier family 22 member 2
• NCBI Official Synonym Full Names: solute carrier family 22 member 2
• NCBI Official Symbol: SLC22A2
• NCBI Official Synonym Symbols: OCT2
• NCBI Protein Information: solute carrier family 22 member 2
• UniProt Protein Name: Solute carrier family 22 member 2
• Protein Family: Solute carrier family
• UniProt Gene Name: SLC22A2
• UniProt Synonym Gene Names: OCT2; hOCT2
• UniProt Entry Name: S22A2_HUMAN

NCBI Description

Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. This gene is one of three similar cation transporter genes located in a cluster on chromosome 6. The encoded protein contains twelve putative transmembrane domains and is a plasma integral membrane protein. It is found primarily in the kidney, where it may mediate the first step in cation reabsorption. [provided by RefSeq, Jul 2008]

Uniprot Description

SLC22A2: Mediates tubular uptake of organic compounds from circulation. Mediates the influx of agmatine, dopamine, noradrenaline (norepinephrine), serotonin, choline, famotidine, ranitidine, histamin, creatinine, amantadine, memantine, acriflavine, 4-[4-(dimethylamino)-styryl]-N-methylpyridinium ASP, amiloride, metformin, N-1-methylnicotinamide (NMN), tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP), cimetidine, cisplatin and oxaliplatin. Cisplatin may develop a nephrotoxic action. Transport of creatinine is inhibited by fluoroquinolones such as DX-619 and LVFX. This transporter is a major determinant of the anticancer activity of oxaliplatin and may contribute to antitumor specificity. Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: basolateral plasma membrane; integral to plasma membrane; membrane; plasma membrane

Molecular Function: choline transmembrane transporter activity; dopamine transmembrane transporter activity; neurotransmitter transporter activity; organic cation transmembrane transporter activity; quaternary ammonium group transmembrane transporter activity; steroid binding

Biological Process: body fluid secretion; choline transport; dopamine transport; histamine transport; multidrug transport; neurotransmitter biosynthetic process; neurotransmitter secretion; organic cation transport; quaternary ammonium group transport