Rabbit anti-Human SLC12A5 Polyclonal Antibody | anti-SLC12A5 antibody

SLC12A5, NT (SLC12A5, KCC2, KIAA1176, Solute carrier family 12 member 5, Electroneutral potassium-chloride cotransporter 2, K-Cl cotransporter 2, Neuronal K-Cl cotransporter) (Azide free) (HRP)

Cat. Num. AAA6348026

• Gene Names: SLC12A5; KCC2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SLC12A5; Polyclonal Antibody; NT (SLC12A5; KCC2; KIAA1176; Solute carrier family 12 member 5; Electroneutral potassium-chloride cotransporter 2; K-Cl cotransporter 2; Neuronal K-Cl cotransporter) (Azide free) (HRP); Neuronal K-Cl cotransporter; anti-SLC12A5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SLC12A5 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SLC12A5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-41 amino acids from the N-terminal region of human SLC12A5.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SLC12A5 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SLC12A5 antibody detected the SLC12A5 protein (arrow).)

Immunohistochemistry (IHC)

(SLC12A5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SLC12A5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-SLC12A5 antibody

Antibodies; KIAA; orf

NCBI and Uniprot Product Information

• NCBI GI #: 11968148
• NCBI GeneID: 57468
• NCBI Accession #: NP_065759.1; NP_001128243.1
• NCBI GenBank Nucleotide #: NM_020708.4
• UniProt Accession #: Q9H2X9; Q5VZ41; Q9H4Z0; Q9ULP4; A2RTX2
• Molecular Weight: 123,511 Da
• NCBI Official Full Name: solute carrier family 12 member 5 isoform 2
• NCBI Official Synonym Full Names: solute carrier family 12 (potassium/chloride transporter), member 5
• NCBI Official Symbol: SLC12A5
• NCBI Official Synonym Symbols: KCC2
• NCBI Protein Information: solute carrier family 12 member 5; K-Cl cotransporter 2; electroneutral potassium-chloride cotransporter 2; erythroid K-Cl cotransporter 2; hKCC2; neuronal K-Cl cotransporter
• UniProt Protein Name: Solute carrier family 12 member 5
• Protein Family: Solute carrier family
• UniProt Gene Name: SLC12A5
• UniProt Synonym Gene Names: KCC2; KIAA1176; hKCC2
• UniProt Entry Name: S12A5_HUMAN

NCBI Description

K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Sep 2008]

Uniprot Description

KCC2: Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons. Homomultimer and heteromultimer with other K-Cl cotransporters. Interacts with AP2A1. Brain specific. Detected in neuronal cells. Inhibited by WNK3. Belongs to the SLC12A transporter family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, SLC family; Membrane protein, multi-pass; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: cell soma; integral to plasma membrane; integral to membrane; plasma membrane; dendritic shaft

Molecular Function: protein kinase binding; potassium:chloride symporter activity

Biological Process: response to drug; synaptic transmission; transport; cellular ion homeostasis; multicellular organism growth; ion transport; learning; transmembrane transport; potassium ion transport; thermosensory behavior