Immunohistochemistry (IHC)
(Immunochemical staining of mouse SLAMF8 in mouse spleen with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
Rabbit anti-Mouse SLAMF8 Polyclonal Antibody | anti-SLAMF8 antibody
Anti-SLAMF8 Antibody, Rabbit Polyclonal
Gene Names
Slamf8; Blame; SBBI42; 5830408F06Rik
Reactivity
Mouse
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
SLAMF8; Polyclonal Antibody; Anti-SLAMF8 Antibody; Rabbit Polyclonal; SLAMF8 Antibody; Rabbit PAb; Antigen Affinity Purified; SLAM family member 8; Anti-5830408F06Rik Antibody; Anti-Blame Antibody; Anti-SBBI42 Antibody; anti-SLAMF8 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse SLAMF8
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-SLAMF8 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
IHC-P: 1:500-1:2000
ELISA: 1:5000-1:10,000
IHC-P: 1:500-1:2000
Immunogen
Recombinant Mouse SLAMF8 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse SLAMF8 (rMouse SLAMF8). SLAMF8 specific IgG was purified by Mouse SLAMF8 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of mouse SLAMF8 in mouse spleen with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse SLAMF8 in mouse spleen with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
Western Blot (WB)
(Anti-SLAMF8 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:32 kDaObserved band size:27 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-SLAMF8 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:32 kDaObserved band size:27 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-SLAMF8 antibody
The signaling lymphocyte activation molecule (SLAM) family includes homophilic and heterophilic receptors that modulate both adaptive and innate immune responses. These receptors share a common ectodomain organization: a membrane-proximal immunoglobulin constant domain and a membrane-distal immunoglobulin variable domain that is responsible for ligand recognition. SLAM family of receptors is expressed by a wide range of immune cells. Through their cytoplasmic domain, SLAM family receptors associate with SLAM-associated protein (SAP)-related molecules, a group of cytoplasmic adaptors composed almost exclusively of an SRC homology 2 domain. SLAM family receptors, in association with SAP family adaptors, have crucial roles during normal immune reactions in innate and adaptive immune cells.
Mouse SLAM family member 8, also known as B-lymphocyte activator macrophage expressed, BCM-like membrane protein, SLAMF8 and BLAME, is a single-pass type I membrane protein. It contains one Ig-like C2-type (immunoglobulin-like) domain. SLAMF8/BLAME is expressed in lymph node, spleen, thymus and bone marrow. It may play a role in B-lineage commitment and/or modulation of signaling through the B-cell receptor.
Mouse SLAM family member 8, also known as B-lymphocyte activator macrophage expressed, BCM-like membrane protein, SLAMF8 and BLAME, is a single-pass type I membrane protein. It contains one Ig-like C2-type (immunoglobulin-like) domain. SLAMF8/BLAME is expressed in lymph node, spleen, thymus and bone marrow. It may play a role in B-lineage commitment and/or modulation of signaling through the B-cell receptor.
Product Categories/Family for anti-SLAMF8 antibody
References
Kingsbury G.A., et al., 2001, J. Immunol. 166:5675-80. Zhang Z., et al., 2004, Protein Sci. 13: 2819-24. Veillette, A. et al., 2006, Nat Rev Immunol 6 (1): 56-66. Veillette, A. et al., 2006, Trends Immunol 27 (5): 228-34. Yan, Q. et al., 2007, Proc Natl Acad Sci. USA.104 (25):10583-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,646 Da
NCBI Official Full Name
SLAM family member 8
NCBI Official Synonym Full Names
SLAM family member 8
NCBI Official Symbol
Slamf8
NCBI Official Synonym Symbols
Blame; SBBI42; 5830408F06Rik
NCBI Protein Information
SLAM family member 8; B lymphocyte activator macrophage expressed; B-lymphocyte activator macrophage expressed
UniProt Protein Name
SLAM family member 8
Protein Family
UniProt Gene Name
Slamf8
UniProt Synonym Gene Names
Blame
UniProt Entry Name
SLAF8_MOUSE
Uniprot Description
Function: May play a role in B-lineage commitment and/or modulation of signaling through the B-cell receptor. Ref.1
Subcellular location: Membrane; Single-pass type I membrane protein.
Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Research Articles on SLAMF8
Similar Products
Product Notes
The SLAMF8 slamf8 (Catalog #AAA8111838) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SLAMF8 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SLAMF8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IHC-P: 1:500-1:2000. Researchers should empirically determine the suitability of the SLAMF8 slamf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLAMF8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
