Testing Data
(PBMC activated with IFN-g were stained with MBS602457 or isotype control open histogram) followed by PE-conjugated BLAME)
Mouse anti-Human BLAME Monoclonal Antibody | anti-Slamf8 antibody
BLAME (B-lymphocyte Activator Macrophage Expressed)
Gene Names
Slamf8; Blame; SBBI42; 5830408F06Rik; Slamf8
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
PurifiedPurified by Protein G affinity chromatography.
Synonyms
BLAME; Monoclonal Antibody; BLAME (B-lymphocyte Activator Macrophage Expressed); Anti -BLAME (B-lymphocyte Activator Macrophage Expressed); anti-Slamf8 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8H128
Specificity
Recognizes human BLAME. Does not react with recombinant human SLAMF7 or recombinant human SLAMF6.
Purity/Purification
Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Applicable Applications for anti-Slamf8 antibody
ELISA (EL/EIA), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry and ELISA.
Dilution: Flow Cytometry: 25ug/ml. Use 10ul to label 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul.
Direct ELISA: 0.5-1.0ug/ml. Detection limit is ~5ng/well.
Dilution: Flow Cytometry: 25ug/ml. Use 10ul to label 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul.
Direct ELISA: 0.5-1.0ug/ml. Detection limit is ~5ng/well.
Immunogen
NS0-derived recombinant human BLAME extracellular domain (aa23-233; Accession # Q9P0V8)
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(PBMC activated with IFN-g were stained with MBS602457 or isotype control open histogram) followed by PE-conjugated BLAME)
Testing Data
(PBMC activated with IFN-g were stained with MBS602457 or isotype control open histogram) followed by PE-conjugated BLAME)
Related Product Information for anti-Slamf8 antibody
B Lymphocyte Activator Macrophage-Expressed (BLAME), also known as SLAMF8, is an Ig superfamily protein that is part of the CD2 family of receptors. It is expressed on macrophages and dendritic cells. BLAME modulates signaling through the B-cell receptor and may also function during B-lineage commitment.
Product Categories/Family for anti-Slamf8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,562 Da
NCBI Official Full Name
BLAME
NCBI Official Synonym Full Names
SLAM family member 8
NCBI Official Symbol
Slamf8
NCBI Official Synonym Symbols
Blame; SBBI42; 5830408F06Rik; Slamf8
NCBI Protein Information
SLAM family member 8; B lymphocyte activator macrophage expressed; B-lymphocyte activator macrophage expressed
UniProt Protein Name
BLAME
Protein Family
UniProt Gene Name
Slamf8
UniProt Synonym Gene Names
Blame
UniProt Entry Name
Q18PH1_MOUSE
Uniprot Description
SLAMF8: May play a role in B-lineage commitment and/or modulation of signaling through the B-cell receptor. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: membrane; cell; integral to membrane; intracellular
Molecular Function: receptor activity
Biological Process: regulation of NAD(P)H oxidase activity; regulation of kinase activity; defense response to bacterium
Similar Products
Product Notes
The Slamf8 slamf8 (Catalog #AAA602457) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BLAME (B-lymphocyte Activator Macrophage Expressed) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BLAME can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry and ELISA. Dilution: Flow Cytometry: 25ug/ml. Use 10ul to label 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul. Direct ELISA: 0.5-1.0ug/ml. Detection limit is ~5ng/well. Researchers should empirically determine the suitability of the Slamf8 slamf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BLAME, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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