Mouse Advanced Glycosylation End Product Specific Receptor (AGER) RTU ELISA Kit | AGER rtu elisa kit

Mouse Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit

Cat. Num. AAA457474

• Gene Names: Ager; RAGE
• Reactivity: Mouse
• Synonyms: Advanced Glycosylation End Product Specific Receptor (AGER); Mouse Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit; RAGE; Receptor For Advanced Glycation Endproducts; AGER rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 393

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.058ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AGER rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AGER. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to AGER. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AGER, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AGER in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 406855404
• NCBI GeneID: 11596
• NCBI Accession #: NP_001258351.1
• NCBI GenBank Nucleotide #: NM_001271422.1
• UniProt Accession #: Q62151; C5H3H4; C5H3H5; C5H3H7; C5H3I0; C5H7W3; C5H7W4; C5H7W5; C5H7W6; C5H7W7; C5H7W8; C5H7W9
• Molecular Weight: 3,036 Da
• NCBI Official Full Name: advanced glycosylation end product-specific receptor isoform b
• NCBI Official Synonym Full Names: advanced glycosylation end product-specific receptor
• NCBI Official Symbol: Ager
• NCBI Official Synonym Symbols: RAGE
• NCBI Protein Information: advanced glycosylation end product-specific receptor
• UniProt Protein Name: Advanced glycosylation end product-specific receptor
• Protein Family: Advanced glycosylation end product-specific receptor
• UniProt Gene Name: Ager
• UniProt Synonym Gene Names: Rage

Uniprot Description

Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Can also bind oligonucleotides. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. RAGE-dependent signaling in microglia contributes to neuroinflammation, amyloid accumulation, and impaired learning/memory in a mouse model of Alzheimer disease.