Rabbit anti-Human SLAMF7 Polyclonal Antibody | anti-SLAMF7 antibody

SLAMF7 Antibody

Cat. Num. AAA9629668

• Gene Names: SLAMF7; 19A; CS1; CD319; CRACC
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: SLAMF7; Polyclonal Antibody; SLAMF7 Antibody; 19A; 19A24 protein; CD 319; CD2 like receptor activating cytotoxic cells; CD2 subset 1; CD319; CD319 antigen; CRACC; CS1; Membrane protein FOAP 12; Novel Ly9; Novel LY9 (lymphocyte antigen 9) like protein; Protein 19A; SLAM family member 7; SLAMF 7; anti-SLAMF7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: SLAMF7 Antibody detects endogenous levels of total SLAMF7.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-SLAMF7 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: The ITSMs (immunoreceptor tyrosine-based switch motifs) with the consensus sequence T-X-Y-X-X-[VI] present in SLAM family receptors have overlapping specificity for activating and inhibitory SH2 domain-containing binding partners. Especially they mediate the interaction with the SH2 domain of SH2D1A and SH2D1B. A 'three-pronged' mechanism is proposed involving threonine (position -2), phosphorylated tyrosine (position 0) and valine/isoleucine (position +3).
• Subunit Structure: Isoform 1 binds to SH2D1A when its cytoplasmic tail is phosphorylated in the presence of FYN (in vitro); low affinity binding, the physiological relevance of the interaction is questioned. Interacts with SH2D1B; in NK cells. Interacts (via ITSM phosphorylated on Tyr-302) with SH2D1B, PTPN6/SHP-1, PTPN11/SHP-2, INPP5D/SHIP1, CSK and FYN (By similarity).
• Tissue Specificity: Expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Expression was detected in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-, or T-cell lines. Expressed in monocytes. Isoform 3 is expressed at much lower level than isoform 1.
• Function: Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Isoform 1 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway. Positively regulates NK cell functions by a mechanism dependent on phosphorylated SH2D1B. Downstream signaling implicates PLCG1, PLCG2 and PI3K. In addition to heterotypic NK cells-target cells interactions also homotypic interactions between NK cells may contribute to activation. However, in the absence of SH2D1B, inhibits NK cell function. Acts also inhibitory in T-cells (By similarity). May play a role in lymphocyte adhesion. In LPS-activated monocytes negatively regulates production of proinflammatory cytokines.Isoform 3 does not mediate any NK cell activation.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-SLAMF7 antibody

Membrane; Single-pass type I membrane protein; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 543583691
• NCBI GeneID: 57823
• NCBI Accession #: NP_001269517.1
• NCBI GenBank Nucleotide #: NM_001282588.1
• UniProt Accession #: Q9NQ25; Q8N6Y8; Q8ND32; Q9NY08; Q9NY23; A8K3U1; B4DPU4; B4DPY3; B4DWA3
• Molecular Weight: 21,316 Da
• NCBI Official Full Name: SLAM family member 7 isoform b
• NCBI Official Synonym Full Names: SLAM family member 7
• NCBI Official Symbol: SLAMF7
• NCBI Official Synonym Symbols: 19A; CS1; CD319; CRACC
• NCBI Protein Information: SLAM family member 7
• UniProt Protein Name: SLAM family member 7
• Protein Family: SLAM family
• UniProt Gene Name: SLAMF7
• UniProt Synonym Gene Names: CS1; CRACC
• UniProt Entry Name: SLAF7_HUMAN

Uniprot Description

SLAMF7: Isoform 1 mediates NK cell activation through a SH2D1A- independent extracellular signal-regulated ERK-mediated pathway. May play a role in lymphocyte adhesion. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.1-q24.1

Cellular Component: plasma membrane

Biological Process: regulation of immune response