Mouse TARBP2 Monoclonal Antibody | anti-TARBP2 antibody

TARBP2 (TAR (HIV-1) RNA Binding Protein 2, LOQS, TRBP, TRBP1, TRBP2) (FITC)

Cat. Num. AAA6177250

• Gene Names: TARBP2; LOQS; TRBP; TRBP1; TRBP2
• Applications: Western Blot
• Purity: Purified
• Synonyms: TARBP2; Monoclonal Antibody; TARBP2 (TAR (HIV-1) RNA Binding Protein 2; LOQS; TRBP; TRBP1; TRBP2) (FITC); TAR (HIV-1) RNA Binding Protein 2; TRBP2; anti-TARBP2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2H1
• Specificity: Recognizes TARBP2.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
• Sequence Length: 366

• Applicable Applications for anti-TARBP2 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: TARBP2 (NP_599150, 141aa-250aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.; ; FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TARBP2 monoclonal antibody (M02), clone 2H1. Western Blot analysis of TARBP2 expression in Jurkat (Cat # L017V1).)

Testing Data

(Detection limit for recombinant GST tagged TARBP2 is approximately 0.3ng/ml as a capture antibody.)

Western Blot (WB)

(TARBP2 monoclonal antibody (M02), clone 2H1. Western Blot analysis of TARBP2 expression in human kidney.)

Related Product Information for anti-TARBP2 antibody

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene. [provided by RefSeq]

Product Categories/Family for anti-TARBP2 antibody

Antibodies; Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 19743840
• NCBI GeneID: 6895
• NCBI Accession #: NP_599150
• NCBI GenBank Nucleotide #: NP_599150.1
• UniProt Accession #: Q15633
• NCBI Official Full Name: RISC-loading complex subunit TARBP2 isoform a
• NCBI Official Synonym Full Names: TARBP2 subunit of RISC loading complex
• NCBI Official Symbol: TARBP2
• NCBI Official Synonym Symbols: LOQS; TRBP; TRBP1; TRBP2
• NCBI Protein Information: RISC-loading complex subunit TARBP2
• UniProt Protein Name: RISC-loading complex subunit TARBP2
• Protein Family: RISC-loading complex
• UniProt Gene Name: TARBP2
• UniProt Entry Name: TRBP2_HUMAN

NCBI Description

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene. [provided by RefSeq, Jul 2008]

Uniprot Description

TRBP: the HIV-1 TAR RNA binding protein (TRBP) is a double strand RNA binding protein (dsRBP) that is required for the recruitment of Ago2 to the small interfering RNA (siRNA) bound by Dicer. TRBP increases HIV-1 expression and replication by inhibiting the interferon-induced protein kinase PKR and by increasing translation of viral mRNA. Dicer, Ago2, and TRBP are the main components of the RNA-induced silencing complex (RISC).

Protein type: Translation; Motility/polarity/chemotaxis; RNA processing; RNA-binding

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; siRNA binding; protein homodimerization activity; miRNA binding; double-stranded RNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; regulation of translation; positive regulation of viral genome replication; regulation of transcription, DNA-dependent; pre-microRNA processing; regulation of viral transcription; RNA interference, targeting of mRNA for destruction; miRNA-mediated gene silencing, miRNA loading onto RISC; negative regulation of protein kinase activity; gene expression; negative regulation of defense response to virus by host; RNA interference, production of siRNA