Testing Data #2
(alamarBlueR indicator dye (MBS238968) quantitatively measures proliferation in human, animal, bacterial, fungal and mycobacterial cells. It is useful for cytokine bioassays, cell proliferation studies and in-vitro cytotoxicity determinations as well as cell growth monitoring)
alamarBlue Accessory Reagent
alamarBlue
Applications
ELISA, Functional Assay, Immunofluorescence
Synonyms
alamarBlue; alamarBlue accessory reagent
Form/Format
Reagent
Liquid
Liquid
Applicable Applications for alamarBlue accessory reagent
ELISA (EIA), Functional Assays (FN), Immunofluorescence (IF)
Intended Use
Cell proliferation assays.
The bioassay can be used to establish proliferation or relative cytotoxicity.
Baseline data for predicting the toxicity of related novel agents can be compared to baseline data with known in-vivo toxicity.
alamarBlue is for use between pH6.8 and pH7.4.
The bioassay can be used to establish proliferation or relative cytotoxicity.
Baseline data for predicting the toxicity of related novel agents can be compared to baseline data with known in-vivo toxicity.
alamarBlue is for use between pH6.8 and pH7.4.
Preparation and Storage
Store at 4 degree C. Dot not Freeze!
This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Testing Data #2
(alamarBlueR indicator dye (MBS238968) quantitatively measures proliferation in human, animal, bacterial, fungal and mycobacterial cells. It is useful for cytokine bioassays, cell proliferation studies and in-vitro cytotoxicity determinations as well as cell growth monitoring)
Testing Data #2
(alamarBlueR indicator dye (MBS238968) quantitatively measures proliferation in human, animal, bacterial, fungal and mycobacterial cells. It is useful for cytokine bioassays, cell proliferation studies and in-vitro cytotoxicity determinations as well as cell growth monitoring)
Related Product Information for alamarBlue accessory reagent
Description: The cell proliferation assay reagent alamarBlue is designed to provide a rapid and sensitive measure of cell proliferation and cytotoxicity in various human and animal cell lines, bacteria and fungi.
Some variability in the absorbance may occur between batches of AlamarBlue(R) but all batches should fall between 0.84 and 95AU when measured between 600nm and 602nm on a spectrophotometer.
Full cell proliferation assay instructions can be found at www.abdMyBioSource.com/uploads/IFU/MBS238967.pdf For further information and Technical help about alamarBlue, the cell proliferation assay reagent, please visit www.abdMyBioSource.com/alamarBlueThis site includes: Frequently Asked QuestionsExample calculationsProduct-related references
Test Principle: Cell proliferation assay Growing cells cause a chemical reduction of alamarBlue.
Continued growth maintains a reduced environment. (fluorescent, red).
Inhibition of growth maintains an oxidized environment. (non-fluorescent, blue).
Data may be collected using either fluorescence-based or absorbance-based instrumentation.
Fluorescence is monitored at 530-560nm excitation wavelength and 590nm emission wavelength.
Absorbance is monitored at 570nm and 600nm.
Some variability in the absorbance may occur between batches of AlamarBlue(R) but all batches should fall between 0.84 and 95AU when measured between 600nm and 602nm on a spectrophotometer.
Full cell proliferation assay instructions can be found at www.abdMyBioSource.com/uploads/IFU/MBS238967.pdf For further information and Technical help about alamarBlue, the cell proliferation assay reagent, please visit www.abdMyBioSource.com/alamarBlueThis site includes: Frequently Asked QuestionsExample calculationsProduct-related references
Test Principle: Cell proliferation assay Growing cells cause a chemical reduction of alamarBlue.
Continued growth maintains a reduced environment. (fluorescent, red).
Inhibition of growth maintains an oxidized environment. (non-fluorescent, blue).
Data may be collected using either fluorescence-based or absorbance-based instrumentation.
Fluorescence is monitored at 530-560nm excitation wavelength and 590nm emission wavelength.
Absorbance is monitored at 570nm and 600nm.
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Product Notes
The alamarBlue (Catalog #AAA238968) is an Accessory Reagent and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's alamarBlue can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Functional Assays (FN), Immunofluorescence (IF). Researchers should empirically determine the suitability of the alamarBlue for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alamarBlue, Accessory Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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