Rabbit SkiP Polyclonal Antibody | anti-SNW1 antibody

SkiP Antibody

Cat. Num. AAA150270

• Gene Names: SNW1; Bx42; SKIP; Prp45; SKIIP; PRPF45; NCOA-62
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: SkiP Antibody is affinity chromatography purified via peptide column.
• Synonyms: SkiP; Polyclonal Antibody; SkiP Antibody; Bx42; SKIP; Prp45; SKIIP; PRPF45; NCOA-62; SNW domain-containing protein 1; Nuclear protein SkiP; SNW domain containing 1; anti-SNW1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: SkiP Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Sequence Length: 536

• Applicable Applications for anti-SNW1 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: SkiP antibody can be used for detection of SkiP by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: SkiP antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human SkiP.
• Buffer: SkiP Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: SkiP antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of SkiP in mouse skeletal muscle tissue lysate with SkiP antibody at 0.5 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of Ski in human kidney tissue with Ski antibody at 20 μg/mL.)

Related Product Information for anti-SNW1 antibody

SkiP Antibody: TGF-beta and the bone morphogenic proteins (BMPs) are key signaling proteins that regulate numerous cellular processes such as embryonic development and tumorigenesis. Both signal through the Smad protein family and are negatively regulated by Ski and SnoN, two related proto-oncoproteins. Ski functions by binding to the Smad proteins activated by TGF-beta and the (BMPs) and preventing their phosphorylation, inhibiting their ability to bind DNA and activate the transcription of downstream genes. SkiP was originally identified as a Ski-interacting protein and was later found to augment the signals induced by TGF-beta but inhibit transcription induced by BMP-2 in C2C12 cells, suggesting that SkiP is a key player in the signaling cascades inititated by TGF-beta and the BMP protein family.

NCBI and Uniprot Product Information

• NCBI GI #: 6912676
• NCBI GeneID: 22938
• NCBI Accession #: NP_036377
• NCBI GenBank Nucleotide #: NM_012245.2
• UniProt Accession #: Q13573; Q13483; Q32N03; Q5D0D6; A8K8A9
• Molecular Weight: 61,494 Da
• NCBI Official Full Name: SNW domain-containing protein 1
• NCBI Official Synonym Full Names: SNW domain containing 1
• NCBI Official Symbol: SNW1
• NCBI Official Synonym Symbols: Bx42; SKIP; Prp45; SKIIP; PRPF45; NCOA-62
• NCBI Protein Information: SNW domain-containing protein 1; nuclear protein SkiP; SKI interacting protein; SKI-interacting protein; homolog of Drosophila BX42; nuclear receptor coactivator, 62-kD; nuclear receptor coactivator NCoA-62
• UniProt Protein Name: SNW domain-containing protein 1
• Protein Family: SNW domain-containing protein
• UniProt Gene Name: SNW1
• UniProt Synonym Gene Names: SKIIP; SKIP
• UniProt Entry Name: SNW1_HUMAN

NCBI Description

This gene, a member of the SNW gene family, encodes a coactivator that enhances transcription from some Pol II promoters. This coactivator can bind to the ligand-binding domain of the vitamin D receptor and to retinoid receptors to enhance vitamin D-, retinoic acid-, estrogen-, and glucocorticoid-mediated gene expression. It can also function as a splicing factor by interacting with poly(A)-binding protein 2 to directly control the expression of muscle-specific genes at the transcriptional level. Finally, the protein may be involved in oncogenesis since it interacts with a region of SKI oncoproteins that is required for transforming activity. [provided by RefSeq, Jul 2008]

Uniprot Description

Skip: Involved in vitamin D-mediated transcription. Can function as a splicing factor in pre-mRNA splicing. Belongs to the SNW family.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor; Spliceosome

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: nucleoplasm; spliceosome; transcription elongation factor complex b; nuclear matrix; chromatin; nucleus

Molecular Function: protein binding; vitamin D receptor binding; retinoic acid receptor binding; transcription coactivator activity; Notch binding; SMAD binding; transcription corepressor activity; nuclear hormone receptor binding

Biological Process: retinoic acid receptor signaling pathway; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; positive regulation of neurogenesis; viral reproduction; positive regulation of transforming growth factor beta receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; positive regulation of nuclear mRNA splicing, via spliceosome; nuclear mRNA splicing, via spliceosome; regulation of transcription from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; regulation of retinoic acid receptor signaling pathway